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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab31474? Please let us know so that we can cite the reference in this datasheet
ab31474 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Paraffin-embedded sections) - NFkB p100 / p52 antibody (ab31474)
Ab31474, at a dilution of 1/50, staining NFkB p100 / p52 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: treated with ab31474.
Right image: treated with the same antibody preincubated with sythesized peptide.
Western blot - NFkB p100 / p52 (phospho S865) antibody (ab31474)
All lanes : Anti-NFkB p100 / p52 (phospho S865) antibody (ab31474) at 1/500 dilution
Lane 1 : Extracts of ovary cancer cells (5-30ug).
Lane 2 : Extracts of ovary cancer cells (5-30ug). Antibody pre-incubated with synthesized peptide.
Secondary
goat-anti-rabbit IgG-AP-conjugate.
Predicted band size : 54 kDa
Immunocytochemistry/ Immunofluorescence - NFkB p100 / p52 (phospho S865) antibody (ab31474)
ICC/IF image of ab31474 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31474, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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