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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab31475? Please let us know so that we can cite the reference in this datasheet
ab31475 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Paraffin-embedded sections) - NFkB p100 / p52 antibody (ab31475)
Ab31475, at a dilution of 1/50-1/100, staining NFkB p100 / p52 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: Untreated.
Right image: Antibody pre incubated with synthesized phosphopeptide.
Western blot - NFkB p100 / p52 (phospho S869) antibody (ab31475)
All lanes : Anti-NFkB p100 / p52 (phospho S869) antibody (ab31475) at 1/500 dilution
Lane 1 : Extracts of Hela cells untreated (5-30ug).
Lane 2 : Extracts of MOLT cells untreated (5-30ug).
Lane 3 : Extracts of MDA-MB-435 cells treated with TNF alpha (5-30ug).
Secondary
goat-anti-rabbit IgG-AP-conjugate.
Predicted band size : 54 , 97 kDa
Observed band size : 67,105 kDa (why is the actual band size different from the predicted?)
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