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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Immunohistochemistry (Paraffin-embedded sections) - NFkB p100 / p52 antibody (ab31475)
Ab31475, at a dilution of 1/50-1/100, staining NFkB p100 / p52 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: Untreated.
Right image: Antibody pre incubated with synthesized phosphopeptide.
Western blot - NFkB p100 / p52 (phospho S869) antibody (ab31475)
All lanes : Anti-NFkB p100 / p52 (phospho S869) antibody (ab31475) at 1/500 dilution
Lane 1 : Extracts of Hela cells untreated (5-30ug).
Lane 2 : Extracts of MOLT cells untreated (5-30ug).
Lane 3 : Extracts of MDA-MB-435 cells treated with TNF alpha (5-30ug).
Secondary
goat-anti-rabbit IgG-AP-conjugate.
Predicted band size : 54 , 97 kDa
Observed band size : 67,105 kDa (why is the actual band size different from the predicted?)
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