Anti-NFkB p105 / p50 (phospho S337) antibody (ab28849)

Overview

• Product nameAnti-NFkB p105 / p50 (phospho S337) antibodySee all NFkB p105 / p50 primary antibodies ...
• Description
  Rabbit polyclonal to NFkB p105 / p50 (phospho S337)
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  The antiserum was produced against synthesized phosphopeptide derived from human NFkappa B p105/p50 around the phosphorylation site of Serine 337.

• Positive controlWB: MDA cell extract. IHC-P: breast carcinoma.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide.
  Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4.
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Nuclear signaling
  • NFkB pathway

  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • NFkB Pathway

  • Other Factors

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • NFkB pathway

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Immunology
  • Innate Immunity
  • Macrophage / Inflamm.

  • Cell Biology
  • Apoptosis
  • Intracellular
  • NFkB
  • p50

Applications

Target

• FunctionNF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
• Sequence similaritiesContains 7 ANK repeats.
  Contains 1 death domain.
  Contains 1 RHD (Rel-like) domain.
• DomainThe C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.
  Glycine-rich region (GRR) appears to be a critical element in the generation of p50.
• Post-translational
  modificationsWhile translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p50 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing.
  Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' are required for BTRC/BTRCP-mediated proteolysis.
  Polyubiquitination seems to allow p105 processing.
  S-nitrosylation of Cys-61 affects DNA binding.
• Cellular localizationNucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor.

Target information above from: UniProt accession P19838 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4790 Human
  • Entrez Gene: 18033 Mouse
  • Entrez Gene: 81736 Rat
  • Omim: 164011 Human
  • SwissProt: P19838 Human
  • SwissProt: P25799 Mouse
  • SwissProt: Q63369 Rat
  • Unigene: 618430 Human

  • Unigene: 256765 Mouse
  • Unigene: 2411 Rat

  see all

• Alternative names
  DKFZp686C01211 antibodyDNA binding factor KBF1 antibodyDNA binding factor KBF1 EBP1 antibody

  DNA binding factor KBF1 EBP1 antibodyDNA-binding factor KBF1 antibodyEBP 1 antibodyEBP-1 antibodyEBP1 antibodyKBF1 antibodyMGC54151 antibodyNF kappa B antibodyNF kappabeta antibodyNF kB1 antibodyNFKB 1 antibodyNFKB p105 antibodyNFKB p50 antibodyNFKB1 antibodyNFKB1_HUMAN antibodyNuclear factor kappa B DNA binding subunit antibodyNuclear factor NF kappa B antibodyNuclear factor NF kappa B p105 subunit antibodyNuclear factor NF kappa B p50 subunit antibodyNuclear factor NF-kappa-B p50 subunit antibodyNuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibodyNuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibodyp84/NF-kappa-B1 p98 antibody

  see all

Anti-NFkB p105 / p50 (phospho S337) antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - p105 (phospho S337) antibody (ab28849)
  Ab28849, at a dilution of 1/50, staining p105 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
• 

  Western blot - NFkB p105 / p50 (phospho S337) antibody (ab28849)
  All lanes : Anti-NFkB p105 / p50 (phospho S337) antibody (ab28849)
  
  Lane 1 : Untreated extracts from MDA-MB-435 cells (5-30ug).
  Lane 2 : Extracts from MDA-MB-435 cells (5-30ug) treated with synthesized phosphopeptide.
  
  
  Predicted band size : 106 kDa
  Observed band size : 118 kDa (why is the actual band size different from the predicted?)