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Overview

  • Product nameNFkB p65 peptideSee all NFkB p65 proteins and peptides ...
  • Protein descriptionSynthetic peptide derived from within residues 500 to the C-terminus of Human NFkB p65. (Note: the amino acid sequence is proprietary). 
  • Protein length551 amino acids

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferInformation available upon request.
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab16636 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Blocking
  • Application notesBL: Use at a concentration of 1 µg/ml. This peptide can be used with NFkB p65 antibody (ab16502).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Avian reticuloendotheliosis viral (v rel) oncogene homolog AMGC131774NF kappa B p65delta3
        NFKB 3NFKB3Nuclear Factor NF Kappa B p65 SubunitNuclear factor NF-kappa-B p65 subunitNuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B CellsNuclear factor of kappa light polypeptide gene enhancer in B cells 3Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3OTTHUMP00000233473OTTHUMP00000233474OTTHUMP00000233475OTTHUMP00000233476OTTHUMP00000233900p65p65 NF kappaBp65 NFkBRELATF65_HUMANTranscription Factor p65V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog Av rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65))v rel reticuloendotheliosis viral oncogene homolog A (avian)V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65v-rel reticuloendotheliosis viral oncogene homolog A
      see all
  • FunctionNF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
  • Sequence similaritiesContains 1 RHD (Rel-like) domain.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications    Ubiquitinated, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.
    Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes.
    Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity.
    Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of 'Lys-310'.
  • Cellular localizationNucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.

    • Target information above from: UniProt accession Q04206 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    NFkB p65 peptide images

    • Western blot
      Western blot
      All lanes : Anti-NFkB p65 antibody (ab16502) at 0.5 µg/ml

      Lane 1 : Hela whole cell lysate
      Lane 2 : A431 whole cell lysate
      Lane 3 : Hela whole cell lysate with NFkB p65 peptide (ab16636) at 1 µg/ml
      Lane 4 : A431 whole cell lysate with NFkB p65 peptide (ab16636) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size : 60 kDa
      Observed band size : 64 kDa (why is the actual band size different from the predicted?)

    References for NFkB p65 peptide (ab16636)

    ab16636 has not yet been referenced specifically in any publications.

    Publishing research using ab16636 ? Please let us know so that we can cite the reference in this datasheet

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