![- NG2 antibody [132.38] (ab50009) image](#NG2-Primary-antibodies-ab50009-1.jpg)
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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-NG2 antibody [132.38]
See all NG2 products (12) ...
Description
Mouse monoclonal [132.38] to NG2
Tested applications
WB, IP, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Cell extract of HEK293 cells expressing the D3 domain of rat NG2, corresponding to amino acids 1592-2222.
Positive control
WB: Neonatal rat brain extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This antibody is derived from the hybridoma 132.38 produced by the fusion of mouse myeloma cells (P3XAg8.653) and splenocytes from BALB/c mice.
Clonality
Monoclonal
Clone number
132.38
Isotype
IgG1
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Integrins
Neuroscience >> Neurology process >> Neurogenesis
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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- NG2 antibody [132.38] (ab50009)
![- NG2 antibody [132.38] (ab50009) image](/ps/datasheet/images/50/ab50009/NG2-Primary-antibodies-ab50009-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab50009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml.Predicted molecular weight: 251 kDa.
IP: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC/IF: 1/200(PubMed: 18974869)
Target
Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Tissue specificity
Detected only in malignant melanoma cells.
Sequence similarities
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains.
Post-translational
modifications
O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Cellular localization
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
Target information above from: UniProt accessionQ6UVK1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MELCSPG antibody
- 4732461B14Rik antibody
- AN2 antibody
see all
Database links
- Entrez Gene: 121021 Mouse
- Entrez Gene: 81651 Rat
- SwissProt: Q8VHY0 Mouse
- SwissProt: Q00657 Rat
- Unigene: 41329 Mouse
- Unigene: 10381 Rat
Anti-NG2 antibody [132.38] images:
- NG2 antibody [132.38] (ab50009)
ab50009 staining NG2 in an immunoreactive cell (rat hippocampal) by Immunohistochemistry (PFA fixed).Brains were postfixed (2–6 hours) in 4% PFA and placed in 30% sucrose (2 days) and sectioned (20–30 µm) with a cryostat. ab50009 was used at a 1/200 dilution. A goat anti-mouse-Alexa 594, 1/500, was used as the secondary antibody.
Image from Lin YC et al, PLoS One. 2010 Sep 10;5(9). pii: e12665, Fig 6.
References for Anti-NG2 antibody [132.38] (ab50009)
This product has been referenced in:
- Fumagalli Met al. Phenotypic changes, signaling pathway and functional correlates of GPR17-expressing neural precursor cells during oligodendrocyte differentiation. J Biol Chem : (2011). ICC/IF; Rat.Read more (PubMed: 21209081) »
- Bharadwaj Set al. Characterization of urine-derived stem cells obtained from upper urinary tract for use in cell-based urological tissue engineering. Tissue Eng Part A 17:2123-32 (2011). ICC/IF; Human.Read more (PubMed: 21513463) »
See all 4 publications for this product
Publishing research using ab50009? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"