NG2 antibody [132.38] (ab50009)

Product Name 

NG2 antibody [132.38]

Product type 

Primary antibodies

Description 

Mouse monoclonal [132.38] to NG2

Immunogen 

Cell extract of HEK293 cells expressing the D3 domain of rat NG2, corresponding to amino acids 1592-2222.

Reacts with 
(species key)

Rat

Tested applications 
(see key)

ELISA, ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 251 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Neonatal rat brain extract.

Cellular localization 

Apical cell membrane; Single pass type I membrane protein; Extracellular side. Cell projection; lamellipodium membrane; Single pass type I membrane protein; Extracellular side. Note: Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.

Research areas 

Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Integrins
Neuroscience >> Neurology process >> Neurogenesis
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker

Relevance 

Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. NG2 is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300 kDa and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumours. NG2 also stimulates alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

This antibody is derived from the hybridoma 132.38 produced by the fusion of mouse myeloma cells (P3XAg8.653) and splenocytes from BALB/c mice.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

132.38

Isotype 

IgG1

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for NG2 antibody [132.38] (ab50009)

  This product has been used in:

Lecca D et al. The recently identified P2Y-like receptor GPR17 is a sensor of brain damage and a new target for brain repair. PLoS One 3:e3579 (2008). ICC/IF; Mouse. PubMed: 18974869

If you publish research using ab50009 please let us know so that we can cite the reference on this datasheet.

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