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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-NG2 antibody [9.2.27]
See all NG2 products (12) ...
Description
Mouse monoclonal [9.2.27] to NG2
Specificity
Recognizes mature NG2 core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa (Bumoi et al., 1984).
Tested applications
Electron Microscopy, Blocking, IP, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin SepharoseTM (Morgan et al., 1981).
Positive control
Human melanoma, glioma and proliferating brain endothelial cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
9.2.27
Isotype
IgG2a
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Integrins
Neuroscience >> Neurology process >> Neurogenesis
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab78284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Electron Microscopy
Blocking
IP
Flow Cyt
ICC/IF
Application notes
BL: Use at an assay dependent dilution. Has been used for functional block of melanoma cell spreading, glioma cell proliferation and tumor growth in nude mice.
Immunoelectron microscopy: Use at an assay dependent dilution.
Flow Cyt: Use 1-2µg for 106 cells.
Immunofluorescence microscopy of cells in culture: Use at an assay dependent dilution.
IP: Use 1µg for 106 cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Tissue specificity
Detected only in malignant melanoma cells.
Sequence similarities
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains.
Post-translational
modifications
O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Cellular localization
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
Target information above from: UniProt accessionQ6UVK1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MEL CSPG antibody
- MELCSPG antibody
- 4732461B14Rik antibody
see all
Database links
- Entrez Gene: 1464 Human
- Omim: 601172 Human
- SwissProt: Q6UVK1 Human
- Unigene: 513044 Human
References for Anti-NG2 antibody [9.2.27] (ab78284)
This product has been referenced in:
- Schrappe Met al. Long-term growth suppression of human glioma xenografts by chemoimmunoconjugates of 4-desacetylvinblastine-3-carboxyhydrazide and monoclonal antibody 9.2.27. Cancer Res 52:3838-44 (1992). ICC/IF, Blocking; Human.Read more (PubMed: 1617657) »
- Bumol TFet al. Biosynthetic studies of proteoglycans in human melanoma cells with a monoclonal antibody to a core glycoprotein of chondroitin sulfate proteoglycans. J Biol Chem 259:12733-41 (1984). ICC/IF, Electron Microscopy, IP; Human.Read more (PubMed: 6386801) »
See all 3 publications for this product
Publishing research using ab78284? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"