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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Anti-NG2 antibody - Extracellular domain (ab81104)
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab81104? Please let us know so that we can cite the reference in this datasheet
ab81104 has been referenced in 1 publications.
- Hall-Glenn F et al. CCN2/connective tissue growth factor is essential for pericyte adhesion and endothelial basement membrane formation during angiogenesis. PLoS One 7:e30562 (2012). IHC-P, Flow Cyt; Mouse. PubMed: 22363445
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence-NG2 antibody - Extracellular domain(ab81104)
ICC/IF image of ab81104 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81104, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - NG2 antibody - Extracellular domain (ab81104)
Anti-NG2 antibody - Extracellular domain (ab81104) at 1/500 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 251 kDa
Observed band size : 300 kDa (why is the actual band size different from the predicted?)
Additional bands at : 31 kDa,51 kDa,61 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Human Chondroitin sulfate proteoglycan 4 precursor (NG2) contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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