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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Anti-NG2 antibody - Extracellular domain (ab81104)
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Immunocytochemistry/ Immunofluorescence-NG2 antibody - Extracellular domain(ab81104)
ICC/IF image of ab81104 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81104, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - NG2 antibody - Extracellular domain (ab81104)
Anti-NG2 antibody - Extracellular domain (ab81104) at 1/500 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 251 kDa
Observed band size : 300 kDa (why is the actual band size different from the predicted?)
Additional bands at : 31 kDa,51 kDa,61 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Human Chondroitin sulfate proteoglycan 4 precursor (NG2) contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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