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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-NG2 antibody - Extracellular domain
See all NG2 products (12) ...
Description
Rabbit polyclonal to NG2 - Extracellular domain
Tested applications
ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Synthetic peptide from the extracellular domain of Mouse NG2, conjugated to an immunogenic carrier protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Integrins
Neuroscience >> Neurology process >> Neurogenesis
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/300 - 1/2000.Predicted molecular weight: 251 kDa.
IHC-P
IHC-P: 1/300 - 1/2000.IHC-P: 1/300 - 1/2000.
IHC-Fr
IHC-Fr: 1/300 - 1/2000.IHC-Fr: 1/300 - 1/2000.
Target
Function
Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Tissue specificity
Detected only in malignant melanoma cells.
Sequence similarities
Contains 15 CSPG (NG2) repeats.
Contains 2 laminin G-like domains.
Post-translational
modifications
O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4.
Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.
Cellular localization
Apical cell membrane. Cell projection > lamellipodium membrane. Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes.
Target information above from: UniProt accessionQ6UVK1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MEL CSPG antibody
- MELCSPG antibody
- 4732461B14Rik antibody
see all
Database links
- Entrez Gene: 121021 Mouse
- SwissProt: Q8VHY0 Mouse
- Unigene: 41329 Mouse
Anti-NG2 antibody - Extracellular domain images:
Immunocytochemistry/ Immunofluorescence-NG2 antibody - Extracellular domain(ab81104)
ICC/IF image of ab81104 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81104, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - NG2 antibody - Extracellular domain (ab81104)
Anti-NG2 antibody - Extracellular domain (ab81104) at 1/500 dilution + Skin (Human) Tissue Lysate - adult normal tissue (ab30166) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 251 kDa
Observed band size : 300 kDa (why is the actual band size different from the predicted?)
Additional bands at : 31 kDa,51 kDa,61 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
Human Chondroitin sulfate proteoglycan 4 precursor (NG2) contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-NG2 antibody - Extracellular domain (ab81104)
ab81104 has not yet been referenced specifically in any publications.
Publishing research using ab81104? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"