Anti-NG2 antibody (ab83508)

Dear Abcam orders,
Im looking to order antibody number ab83508. Altoght there is no
reference published it has worked to stain oligodendrocyte progenitors
in mouse brain, only a picture I could find.
Do you have a reference it stains progenitors in brain?
If not could I have a discount and be the first one to try?
Best regards

ANSWER:

Thank you for your enquiry.

I would like to reassre you that this antibody is covered by our 6 month guarantee for IHC-P and IHC-FrFland for Mouse andHuman samples.

For more details regarding our guarantee, please see the following page from our website:
http://www.abcam.com/index.html?pageconfig=abpromise

I am sorry the antibody has not been specifically tested on brain samples. however it would still be covered by our guarnatee for mouse brain, as it has worked succesfully beforefor mouse tissue. However, I am pleased to provide you with a 5% discount off your purchase of this antibody (ab66157), and free shipping for this order which I hope will be helpful. In order to obtain these discounts, please use the following codes when you place the order:

5% Discount code: 5PC-OFF-T7T62
Free shipping code: NO-PORTO-T7T61

Also, you can obtain further reward by submitting an Abreview about this product via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers.

To find out more about our Abreview system and the Abpoints rewards you can obtain, please see the following webpage:

http://www.abcam.com/abreviews

I hope this will be helpful. Thank you for your time and effort.

What does Tissue specificity section mean?
'Detected only in malignant melanoma cells.'
We are staining mouse brain tissue and have some positive staining in localized regions, the staining looks like membrane stain.

ANSWER:

Thank you for your telephone enquiry yesterday afternoon.

I can understand you concerns regarding the specificity statement on the datasheet. I would liketo confirm that this is a statement from the tissue specificity section in Uniprot. We have an agreement with Uniprot to link to the information from their pages for some sections of our datasheets.

As you discussed with me, this is a rather confusing sentence. Because it mentions 'detected' it perhaps gives the impression that this particular antibody detects NG2 only in malignant melanoma cells. However, I can confirm that NG2 is expressed in a range of other tissues, according to the EST expression profile from Unigene. Including brain tissue:

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.513044

I have also found the following publications online describing NG2 expression in mouse brain:

http://dev.biologists.org/content/135/1/145.full

http://www.ncbi.nlm.nih.gov/pubmed/20346197

I can suggest to try a further literature search for more information.

We appreciate your feedback and our datasheets team is currently working on updating this datasheetto make it clearer for customers. If you still have some concerns regarding the results you have, please do not hesitate to contact me with further information. this antibody is covered by our 6 month guarantee in IHC-P and mouse.

If you have any further questions, please do let me know.

For the below antibodies I am looking for both isotype control and positive controls. It would be great if you could give the information for both in case you have them.
ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> Rabbit IgG
ab83508 --> Mouse IgG1
ab102998 --> Rabbit IgG
In the list I was asking for isotype controls, but still the positive controls would be interesting to know, too.
All the best,

ANSWER:

Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, I can provide a range of the IgG: 10-15 mg/ml.

Isotype controls:



can be searched at this site: http://www.abcam.com/index.html?c=718

ab36204, ab48614, ab66219, ab102998 --> Rabbit IgG - ab37406, ab125938, ab128142

ab83508 --> Mouse IgG1 - ab91353, ab27479, ab81032



Positive control:



ab36204 - CRMP4 - It is mainly expressed in heart and skeletal muscle and also strongly expressed in fetal brain and spinal cord (human, mouse or rat),

ab48614 --Ceruloplasmin - It is expressed by the liver and secreted in plasma,

ab66219 -- Oprl1 - Brain (cortex),

ab83508 -- NG2 - It is detected only in malignant melanoma cells, SK-MEL-28 cells,

ab102998 --B3GNT5- only tested in WB - Widely expressed. It is highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum, MDA-MB435 cell line

If you need any further assistance in the future, please do not hesitate to contact me.

Dear Sir or Madam,
We recently bought the following antibodies from your company: ab36204; ab48614; ab66219; ab83508; ab102998. As we would like to test at least four of them on 4%PFA, sucrose fixed brain tissue and include the correct controls I have a few questions:
I received the following list:
ab36204 (Lot: GR42382) --> concentration: N/A 100µl
ab48614 (Lot: GR62244) --> concentration: 0,7699 mg/ml (100µg)
ab66219 (Lot: GR9812) --> concentration: N/A 100µl
ab83508 (Lot: GR38090) --> concentration: 0.5 mg/ml (100µg)
ab102998 (Lot: GR80395) --> concentration 0.25mg/ml (100µg)
In order to include a control with the same antibody concentration as for the primary antibody it would be good to know the concentration of ab36204 and ab66219, too. Could you help me concerning that matter?
Second question, do you have any experience with good control antibodies? I thought the following:
ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> Rabbit IgG
ab83508 --> Mouse IgG1
ab102998 --> Rabbit IgG
I think that is also what you suggested in the data sheets, I just would like to make sure, that I am correct.
I would be very happy about a rapid answer.
Best regards,

ANSWER:

Thank you for your enquiry and your interest in our products.

This is to confirm that we can only provide the concentration for affinity purified antibodies. For whole serum or supernatant we do not quantify the IgG concentration. I have contacted the lab to get the requested information for you concerning ab66219 (Lot: GR9812).

Would you be so kind to clarify if you wish to look for isotype control or positive control for these primary antibodies listed below?

ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> Rabbit IgG
ab83508 --> Mouse IgG1
ab102998 --> Rabbit IgG

I look forward to hearing from you soon. As soon as I have further information on the concentration (ab66219), I will contact you again.

Thank you for the assistance.
We are in the situation that we - with the exception of the antibody against Ceruloplasmin – don’t have any information whether the antibodies work for the perfusion-fixed, sucrose-immersed, cryostate sectioned brains that we will intend to use these antibodies on. Being responsible for the payment I am therefore a bit hesitant considering the only 5% discount to this relatively large order.
Would you be able to provide a larger discount so we could test this bacth of antibodies? If things works as we hope we would of course stick to you as the supplier of those antibodies.

ANSWER:

My colleague Teresa has forwarded your request to our team.

I have checked the datasheets for the PFA- perfusion fixed frozen section application (IHC-FoFr), andthe antibodiesare all not tested for this. This means unfortunately, that we cannot guarantee the outcome.

However,I can offer a discount off a future purchase if you buyab36204, ab66219, ab83508and /or ab102998now, test it in IHC-FoFr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 freeprimary antibodyper tested antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab36204, ab66219, ab83508 and /or ab102998 in IHC-FoFr. I will then send a discount code. This code must be issued before purchasing ab36204, ab66219, ab83508 and /or ab102998 so please wait for my reply before ordering.

2. Purchase ab36204, ab66219, ab83508 and /or ab102998 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-FoFr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab36204, ab66219, ab83508 and /or ab102998 turns out to be unsuitable for IHC-FoFr, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.