Anti-NINL antibody (ab121110)
Key features and details
- Rabbit polyclonal to NINL
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NINL antibody -
Description
Rabbit polyclonal to NINL -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 682-787 of Human NINL.
-
Positive control
- Human cerebellum tissue. ICC/IF: U2-OS cells
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/0.25 - 1/2.
Fixation/Permeabilization: PFA/Triton X-100
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
1/0.25 - 1/2. Fixation/Permeabilization: PFA/Triton X-100
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis. -
Tissue specificity
Expressed in KYSE150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina. -
Sequence similarities
Contains 4 EF-hand domains. -
Domain
The KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway. -
Post-translational
modificationsPhosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin.
Ubiquitinated by the APC/C complex leading to its degradation. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 22981 Human
- Omim: 609580 Human
- SwissProt: Q9Y2I6 Human
- Unigene: 631508 Human
-
Alternative names
- 4930519N13Rik antibody
- dJ691N24.1 antibody
- Gm1004, antibody
see all
Images
-
ab121101 at 1/100 dilution staining NINL in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
Note: moderate cytoplasmic positivity in purkinje cells. -
Immunocytochemistry/ Immunofluorescence analysis of U-2 OS labeling NINL with ab121110. The image shows localization to cytosol & cytokinetic bridge.
Fixation/Permeabilization: PFA/Triton X-100
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab121110 has been referenced in 1 publication.
- Yin L et al. Epidermal gene expression and ethnic pigmentation variations among individuals of Asian, European and African ancestry. Exp Dermatol 23:731-5 (2014). IHC-P ; Human . PubMed: 25055985