The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionInvolved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis.
Tissue specificityExpressed in KYSE150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina.
Sequence similaritiesContains 4 EF-hand domains.
DomainThe KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway.
Post-translational modificationsPhosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin. Ubiquitinated by the APC/C complex leading to its degradation.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm.
ab121101 at 1/100 dilution staining NINL in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry. Note: moderate cytoplasmic positivity in purkinje cells.
References for Anti-NINL antibody (ab121110)
This product has been referenced in:
Yin L et al. Epidermal gene expression and ethnic pigmentation variations among individuals of Asian, European and African ancestry. Exp Dermatol23:731-5 (2014).
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