Overview

  • Product nameAnti-NINL antibody
    See all NINL primary antibodies
  • Description
    Rabbit polyclonal to NINL
  • Tested applicationsIHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 682-787 of Human NINL.

  • Positive control
    • Human cerebellum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInvolved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis.
  • Tissue specificityExpressed in KYSE150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina.
  • Sequence similaritiesContains 4 EF-hand domains.
  • DomainThe KEN and D (destructive) boxes are required for the cell cycle-controlled NINL degradation by the APC/C pathway.
  • Post-translational
    modifications
    Phosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin.
    Ubiquitinated by the APC/C complex leading to its degradation.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930519N13Rik antibody
    • dJ691N24.1 antibody
    • Gm1004, antibody
    • Gm1634 antibody
    • mKIAA0980 antibody
    • Ninein like antibody
    • Ninein-like protein antibody
    • NINL antibody
    • NINL_HUMAN antibody
    • Nlp antibody
    • RGD1306152 antibody
    • RP23-193L22.3 antibody
    • RP4-691N24.1 antibody
    see all

Anti-NINL antibody images

  • ab121101 at 1/100 dilution staining NINL in Paraffin-embedded Human Cerebellum tissue by Immunohistochemistry.
    Note: moderate cytoplasmic positivity in purkinje cells.

References for Anti-NINL antibody (ab121110)

ab121110 has not yet been referenced specifically in any publications.

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