NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab112149)
Overview
- Product nameNIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry)See all Mitochondrial Transmembrane Potential kits ...
- Tests1 x 100 test
- Sample typeAdherent cells, Suspension cells
- Assay typeDirect
- Product overview
ab112149 is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
ab112149 uses our proprietary cationic NIR probe for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. In normal cells, the red fluorescence intensity is increased when the MitoNIR Dye is accumulated in the mitochondria. However, in apoptotic cells, the NIR stain intensity is decreased following the collapse of MMP. Cells stained with NIR Dye can be visualized with a flow cytometer at red excitation and far red emission (FL4 channel).
ab112149 provides all the essential components. ab112149 can be used together with other reagents, such as blue laser excited propidium iodide and for studying cell vitality and apoptosis. ab112149 is optimized for screening apoptosis activators and inhibitors with a flow cytometer.
Visit our FAQs page for tips and troubleshooting. - Notes
ab112149 should be stored dessicated.
- Tested applicationsFlow Cyt, Functional Studies more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components 100 tests 1 kit Assay Buffer 1 x 100ml 1 x 100ml MitoNIR Dye 1 x 500µl 1 x 500µl -
Research Areas
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Alternative names
- mitochondrial membrane potential
Applications
Our Abpromise guarantee covers the use of ab112149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Flow Cyt | Flow Cyt |
| Functional Studies | FuncS |
NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) images
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Flow Cytometry - NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab112149)The decrease in fluorescence intensity of NIR Dye with the addition of FCCP in Jurkat cells. Jurkat cells were loaded with NIR Dye alone (blue line) or in the presence of 50 µM FCCP (red line) for 10 minutes. The fluorescence intensity of NIR Dye was measured with a flow cytometer.
Protocols
References for NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) (ab112149)
ab112149 has not yet been referenced specifically in any publications.
