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ab112150 is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
ab112150 NIR Mitochondria Membrane Potential Assay Kit provides all the essential components with an optimized assay method. This fluorometric assay uses our proprietary cationic MitoNIR Dye for the detection of the mitochondrial membrane potential change in cells. In normal cells, the red fluorescence intensity is increased when NIR Dye is accumulated in the mitochondria. However, in apoptotic cells, NIR stain intensity is decreased following the collapse of MMP. Cells stained with NIR Dye can be monitored fluorimetrically at Em = 660-680 nm with excitation at Ex = 620-640 nm.
ab112150 can be used for screening apoptosis activators and inhibitors. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.
Visit our FAQs page for tips and troubleshooting.
Store ab112150 desiccated.
|Components||5 x 96 tests|
|Assay Buffer A||1 x 50ml|
|Assay Buffer B||1 x 25ml|
|MitoNIR Dye||1 x 250µl|
Our Abpromise guarantee covers the use of ab112150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab112150 has not yet been referenced specifically in any publications.