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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NIR1 antibody (ab21197)
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Product Name
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NIR1 antibody
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Product type
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Primary antibodies
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Description
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Goat polyclonal to NIR1
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Immunogen
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Synthetic peptide: SWARGPPKFESVP, corresponding to C terminal amino acids 962-974 of NIR1.
BLAST 'SWARGPPKFESVP' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 5 µg/ml.
Peptide ELISA: antibody detection limit dilution 1:16,000.
Western Blot: Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1µg/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
Please note that currently we cannot find an explanation in the literature for the band we observed at size of 107kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Heart lysates
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Cellular localization
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Cell Membrane and Plasma membrane
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Research areas
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Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Calcium Binding Proteins
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> IP3R
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Relevance
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NIR1 is calcium ion binding, has phosphatidylinositol transporter activity and receptor tyrosine kinase binding. It catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes. NIR1 binds calcium ions and interacts with PTK2B via its C-terminus. Expression is detected in brain, spleen and ovary.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NIR1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NIR1 antibody images, references, products related to ab21197 and other tools.
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NIR1 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NIR1 antibody (ab21197)
ab21197 at 5ug/ml staining NIR1 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
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Search PubMed (MEDLINE) for references to NIR1
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NIR1 antibody - more information
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Customer reviews (feedback) regarding NIR1 antibody |
Customer FAQs regarding NIR1 antibody |
Protocols for NIR1 antibody |
Price and availability of products related to NIR1 antibody |
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NIR1 antibody - related products:
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NIR1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NIR1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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