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Products:Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Channels >> IP3R
Overview
Product name
Anti-NIR1 antibody
See all NIR1 products (4) ...
Description
Goat polyclonal to NIR1
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide: SWARGPPKFESVP, corresponding to C terminal amino acids 962-974 of NIR1.
SWARGPPKFE SVP
Positive control
Human Heart lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NIR1 antibody (ab21197)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab21197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
IHC-P
WB
Application notes
IHC-P: Use at a concentration of 5 µg/ml.
Peptide ELISA: antibody detection limit dilution 1:16,000.
Western Blot: Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1µg/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
Please note that currently we cannot find an explanation in the literature for the band we observed at size of 107kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes (in vitro) (By similarity). Binds calcium ions.
Tissue specificity
Detected in brain and spleen, and at low levels in ovary.
Involvement in disease
Defects in PITPNM3 are the cause of cone-rod dystrophy type 5 (CORD5) [MIM:600977]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.
Sequence similarities
Belongs to the PtdIns transfer protein family. PI transfer class IIA subfamily.
Contains 1 DDHD domain.
Cellular localization
Endomembrane system.
Target information above from: UniProt accessionQ9BZ71
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- membrane-associated 3 antibody
- CORD5 antibody
- Membrane associated phosphatidylinositol transfer protein 3 antibody
see all
Database links
- Entrez Gene: 83394 Human
- Entrez Gene: 327958 Mouse
- Omim: 608921 Human
- SwissProt: Q9BZ71 Human
- SwissProt: Q3UHE1 Mouse
- Unigene: 183983 Human
- Unigene: 351793 Mouse
Anti-NIR1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NIR1 antibody (ab21197)
ab21197 at 5ug/ml staining NIR1 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
References for Anti-NIR1 antibody (ab21197)
ab21197 has not yet been referenced specifically in any publications.
Publishing research using ab21197? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"