Anti-NIT1 antibody (ab198203)
Key features and details
- Rabbit polyclonal to NIT1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NIT1 antibody
See all NIT1 primary antibodies -
Description
Rabbit polyclonal to NIT1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human NIT1 (internal sequence).
Database link: Q86X76-5 -
Positive control
- Human prostate cancer and liver cancer tissues; Human placenta tissue and fetal liver tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/200 - 1/1000. Predicted molecular weight: 26 kDa.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/200 - 1/1000. Predicted molecular weight: 26 kDa. |
Target
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Function
Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2. -
Tissue specificity
Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
Contains 1 CN hydrolase domain. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 4817 Human
- Entrez Gene: 27045 Mouse
- Entrez Gene: 289222 Rat
- Omim: 604618 Human
- SwissProt: Q86X76 Human
- SwissProt: Q8VDK1 Mouse
- SwissProt: Q7TQ94 Rat
- Unigene: 146406 Human
see all -
Alternative names
- MGC57670 antibody
- NIT1 antibody
- NIT1_HUMAN antibody
see all
Images
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All lanes : Anti-NIT1 antibody (ab198203) at 1/250 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 26 kDa
Exposure time: 2 minutes8% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling NIT1 with ab198203 at a 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling NIT1 with ab198203 at a 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab198203 has not yet been referenced specifically in any publications.