You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-NIT1 antibodySee all NIT1 primary antibodies ...
  • Description
    Rabbit polyclonal to NIT1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 71-120 (VREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKL LEEYTQLAREC) of Human NIT1 (NP_005591).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab98066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionPlay a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. it is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2.
  • Tissue specificityDetected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the UPF0012 family.
    Contains 1 CN hydrolase domain.
  • Cellular localizationCytoplasm. Mitochondrion.

    • Target information above from: UniProt accession Q86X76 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        MGC57670 antibodyNIT1 antibodyNIT1_HUMAN antibody
        Nitrilase 1 antibodyNitrilase homolog 1 antibody
      see all

    Anti-NIT1 antibody images

    • Western blot - NIT1 antibody (ab98066)
      Western blot - NIT1 antibody (ab98066)
      Anti-NIT1 antibody (ab98066) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T Cell Lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 36 kDa

    References for Anti-NIT1 antibody (ab98066)

    ab98066 has not yet been referenced specifically in any publications.

    Publishing research using ab98066 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab98066 Submit a Question for ab98066

    There are currently no Abreviews or Questions for ab98066.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"