Overview
Product nameNKIRAS1 protein (His tag)
Protein descriptionRecombinant full length protein of Human NKIRAS1 (amino acids 1-192) with a N terminal His tag, 212 amino acids with a predicted MWt 23.8 kDa including tag (UniProt Q9NYS0).
Uniprot accessionQ9NYS0
Molecular weight23.800kDa inclusive of tags
Protein length192 amino acids
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab123143 was purified using conventional chromatography.
Mass spectrometryMALDI-TOF
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol
Concentration information loading...
Sequence notesMGSSHHHHHHSSGLVPRGSHMGKGCKVVVCGLLSVGKTAI LEQLLYGNHTIGMEDCETMEDVYMASVETDRGVKEQLHLY DTRGLQEGVELPKHYFSFADGFVLVYSVNNLESFQRVELL KKEIDKFKDKKEVAIVVLGNKIDLSEQRQVDAEVAQQWAK SEKVRLWEVTVTDRKTLIEPFTLLASKLSQPQSKSSFPLP GRKNKGNSNSEN
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab123143
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent concentration. |
| MS |
MS: Use at an assay dependent concentration. |
Application notesThis peptide can be used with studies using ab13666 and ab139272.
Protein info
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Alternative names
2400004O09RikI kappa B interacting Ras like protein 1I-kappa-B-interacting Ras-like protein 1
Kappa B Ras 1Kappa B Ras protein 1Kappa B-Ras protein 1KappaB Ras1KappaB-Ras1KBRAS 1KBRAS1KBRS1_HUMANNF kappa B inhibitor interacting Ras like protein 1NF-kappa-B inhibitor-interacting Ras-like protein 1NFKB inhibitor interacting Ras like 1NFKB inhibitor interacting Ras like protein 1NKIRAS 1nkiras1
see all
FunctionAtypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.
DomainIn contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Leu residues, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.
Cellular localizationCytoplasm.
NKIRAS1 protein (His tag) images
References for NKIRAS1 protein (His tag) (ab123143)
ab123143
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - NKIRAS1 protein (ab123143)12% SDS PAGE, 3 µg of ab123143 loaded.