Overview
Product nameNKIRAS2 protein (His tag)See all NKIRAS2 proteins and peptides ...
Protein descriptionRecombinant full length protein corresponding to amino acids 1-191 of Human NKIRAS2, fused to an N terminal His-tag; 215 amino acids inclusive of tag, MWt 24.0 kDa inclusive of tag (NP_001138399).
Uniprot accessionQ9NYR9
Molecular weight24kDa inclusive of tags
Protein length191 amino acids
Expression hostE. coli
Properties
Purity> 90
% by SDS-PAGE
Purification notesab124599 is purified by using conventional chromatography techniques.
Mass spectrometryMALDI-TOF
FormLiquid
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride
Concentration information loading...
Sequence notesMGSSHHHHHH SSGLVPRGSH MGSHMGKSCK VVVCGQASVG KTSILEQLLY GNHVVGSEMI ETQEDIYVGS IETDRGVREQ VRFYDTRGLR DGAELPRHCF SCTDGYVLVY STDSRESFQR VELLKKEIDK SKDKKEVTIV VLGNKCDLQE QRRVDPDVAQ HWAKSEKVKL WEVSVADRRS LLEPFVYLAS KMTQPQSKSA FPLSRKNKGS GSLDG
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab124599
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
| MS |
MS: Use at an assay dependent dilution. |
Application notesThis peptide can be used with studies using ab153988 and ab154020.
Protein info
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Alternative names
DKFZP434N1526I kappa B interacting Ras like protein 2I-kappa-B-interacting Ras-like protein 2
Kappa B Ras protein 2Kappa B-Ras protein 2KappaB Ras2KappaB-Ras2KBRAS 2KBRAS2KBRS2_HUMANMGC74742NF kappa B inhibitor interacting Ras like protein 2NF-kappa-B inhibitor-interacting Ras-like protein 2NFKB inhibitor interacting Ras like 2NFKB inhibitor interacting Ras like protein 2NKIRAS 2NKIRAS2
see all
FunctionAtypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.
DomainIn contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.
Cellular localizationCytoplasm.
NKIRAS2 protein (His tag) images
References for NKIRAS2 protein (His tag) (ab124599)
ab124599
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
SDS-PAGE - NKIRAS2 protein (His tag) (ab124599)15% SDS-PAGE showing ab124599 (3µg).