Anti-NLRX1 antibody (ab107611)
Key features and details
- Rabbit polyclonal to NLRX1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Horse, Human
- Isotype: IgG
Overview
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Product name
Anti-NLRX1 antibody
See all NLRX1 primary antibodies -
Description
Rabbit polyclonal to NLRX1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Horse, Human
Predicted to work with: Cow, Dog -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 580-630 of Human NLRX1 (NP_078894.2).
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Positive control
- Mouse heart tissue lysate; Human brain tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab107611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 108 kDa.
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IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 5 - 7 µg/ml. Predicted molecular weight: 108 kDa. |
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313). -
Tissue specificity
Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level). -
Sequence similarities
Belongs to the NLRP family.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 539974 Cow
- Entrez Gene: 100071383 Horse
- Entrez Gene: 79671 Human
- Entrez Gene: 270151 Mouse
- Omim: 611947 Human
- SwissProt: Q86UT6 Human
- SwissProt: Q3TL44 Mouse
- Unigene: 524082 Human
see all -
Alternative names
- Caterpiller protein 11.3 antibody
- CLR11.3 antibody
- DLNB26 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue labelling NLRX1 with ab107611 at 10µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
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All lanes : Anti-NLRX1 antibody (ab107611) at 5 µg/ml
Lane 1 : Mouse heart tissue lysate in absence immunizing peptide
Lane 2 : Mouse heart tissue lysate in presence of immunuzing peptide
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Ig HRP
Developed using the ECL technique.
Predicted band size: 108 kDa -
ab107611, at 5µg, staining NLRX1 in formalin-fixed, paraffin-embedded Human brain tissue by immunohistochemisty.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab107611 has been referenced in 2 publications.
- Zhang H et al. NLRX1 Deletion Increases Ischemia-Reperfusion Damage and Activates Glucose Metabolism in Mouse Heart. Front Immunol 11:591815 (2020). PubMed: 33362773
- Stokman G et al. NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity. J Exp Med 214:2405-2420 (2017). PubMed: 28626071