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Western blot - NMDAR2B antibody [NR2B] (ab28373)
![Western blot - NMDAR2B antibody [NR2B] (ab28373)](/ps/datasheet/images/ab28372-2.png)
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Western blot - NMDAR2B antibody [NR2B] (ab28373)
![Western blot - NMDAR2B antibody [NR2B] (ab28373)](/ps/datasheet/images/ab28373_1.jpg)
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Product Name
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NMDAR2B antibody [NR2B]
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See all NMDAR2B antibodies (19)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [NR2B] to NMDAR2B
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Immunogen
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Recombinant fragment (Rat) - corresponding to amino acids 934 - 1457 of NR2B subunit.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects the N-methyl-D-aspartate (NMDA) receptor type 2B. This antibody has been tested in mouse and rat brain in western blotting. It has not been tested in human for Western blotting - only for ICC on transfected HEK cells.
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Tested applications
(see key)
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ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 166 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Mouse brain tissue
ICC: transfected HEK cells
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Cellular localization
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Integral membrane protein.
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Research areas
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Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Neuroscience >> Sensory System >> somatosensory system >> Nociception
Neuroscience >> Neurotransmission >> Receptors / Channels >> Ligand-Gated Ion Channels >> NMDA Receptors
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Relevance
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NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain types of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA).
Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease, epilepsy and ischemic neuronal cell death.
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NMDAR2B antibody [NR2B] (ab28373) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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NR2B
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Protein A Chromatography
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NMDAR2B antibody [NR2B] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NMDAR2B antibody [NR2B] images, references, products related to ab28373 and other tools.
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NMDAR2B antibody [NR2B] images:
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 Western blot - NMDAR2B antibody [NR2B] (ab28373)
NMDAR2B antibody [NR2B] (ab28373) at 2 µg/ml + mouse brain tissue
Predicted band size : 166 kDa
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Western blot - NMDAR2B antibody [NR2B] (ab28373)
Lanes 2 - 4 : NMDAR2B antibody [NR2B] (ab28373) at 1/500 dilution
Lanes 5 - 7 : NMDAR2B antibody [NR2B] (ab28373) at 1/1000 dilution
Lane 2 : As above
Lane 3 : Mouse brain (50ug)
Lane 4 : Rat brain (50ug)
Lane 5 : As above
Lane 6 : Mouse brain (50ug)
Lane 7 : Rat brain (50ug)
Predicted band size : 166 kDa
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Search PubMed (MEDLINE) for references to NMDAR2B
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NMDAR2B antibody [NR2B] - more information
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Customer reviews (feedback) regarding NMDAR2B antibody [NR2B] |
Customer FAQs regarding NMDAR2B antibody [NR2B] |
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NMDAR2B antibody [NR2B] - related products:
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NMDAR2B antibody [NR2B] - other tools:
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Contact Abcam with a Technical Enquiry about NMDAR2B antibody [NR2B] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - NMDAR2B antibody [NR2B] (ab28373)](/index.html?datasheet=28373#ab28372-2.png){kind=small}
![Western blot - NMDAR2B antibody [NR2B] (ab28373)](/index.html?datasheet=28373#ab28373_1.jpg)
