Anti-NMDAR2B (phospho Y1252) antibody (ab18532)
Key features and details
- Rabbit polyclonal to NMDAR2B (phospho Y1252)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NMDAR2B (phospho Y1252) antibody
See all NMDAR2B primary antibodies -
Description
Rabbit polyclonal to NMDAR2B (phospho Y1252) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 166 kDa (predicted molecular weight: 166 kDa). |
Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
Tissue specificity
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
Alternative names
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
Images
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All lanes : ab18532 at 1 mg/ml
Lane 1 : Mouse Brain Tissue Lysate
Lane 2 : Mouse Fore-brain Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Cerebellum Tissue Lysate
Lane 5 : Mouse Hippo-campus Tissue Lysate
Lane 6 : Human Brain Tissue Lysate
Lane 7 : Human Cortex Tissue Lysate
Lane 8 : Human Cerebellum Tissue Lysate
Lane 9 : Human Hippo-campus Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 166 kDa
Additional bands at: 18 kDa, 45 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Lanes 1-3 : Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/ml
Lane 4 : NR2B antibody (positive control)
Lane 1 : mouse cortical extract with Mouse NMDAR2B (unmodified ) peptide (ab19637) at 2 µg/ml
Lane 2 : mouse cortical extract with Mouse NMDAR2B (phospho Y1252) peptide (ab19636) at 2 µg/ml
Lanes 3-4 : mouse cortical extract
Lysates/proteins at 20 µg per lane.
Predicted band size: 166 kDa
Observed band size: 166 kDa
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab18532 has been referenced in 3 publications.
- Herrera-Zamora JM et al. Development-Dependent Changes in the NR2 Subtype of the N-Methyl-D-Aspartate Receptor in the Suprachiasmatic Nucleus of the Rat. J Biol Rhythms 34:39-50 (2019). PubMed: 30654688
- Sánchez-Blázquez P et al. Sigma 1 Receptor Antagonists Inhibit Manic-Like Behaviors in Two Congenital Strains of Mice. Int J Neuropsychopharmacol 21:938-948 (2018). PubMed: 29860313
- Delint-Ramirez I et al. In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93. J Neurosci 30:8162-70 (2010). PubMed: 20554866