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Anti-NNT antibody [8B4BB10 ] (ab110352)

MSCatalog No. MS701

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Overview

Product name

Anti-NNT antibody [8B4BB10 ]
See all NNT products (2) ...

Description

Mouse monoclonal [8B4BB10 ] to NNT

Tested applications

IP, ICC/IF, In-Cell ELISA, IHC-P, WB, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Rat, Cow, Human

Immunogen

Human heart mitochondria

Positive control

Normal Human skin fibroblasts (strain HDFn); Human cerebellum tissue; mitochondria from Human liver, Bovine heart, Rat liver and Mouse liver; HepG2 and HL-60 cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.02% Sodium azide
Constituent: HBS

Concentration

Concentration information loading...

Purity

>95% by SDS-PAGE

Purification notes

Produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Clonality

Monoclonal

Clone number

8B4BB10

Isotype

IgG1

Light chain type

kappa

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation
Signal Transduction >> Metabolism >> Mitochondrial

  • Flow Cytometry - NNT antibody [8B4BB10 ] (ab110352)Flow Cytometry - NNT antibody [8B4BB10 ] (ab110352) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Anti-NNT antibody [8B4BB10 ] (ab110352)Immunocytochemistry/ Immunofluorescence - Anti-NNT antibody [8B4BB10 ] (ab110352) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NNT antibody [8B4BB10 ] (ab110352)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NNT antibody [8B4BB10 ] (ab110352) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab110352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.

Sequence similarities

In the N-terminal section; belongs to the AlaDH/PNT family.
In the C-terminal section; belongs to the PNT beta subunit family.

Cellular localization

Mitochondrion inner membrane.

Target information above from: UniProt accessionQ13423 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • mitochondrial antibody
  • MS701 antibody
  • NAD(P) transhydrogenase antibody
  • NAD(P) transhydrogenase mitochondrial antibody
  • Nicotinamide nucleotide transhydrogenase antibody
  • NNT antibody
  • NNTM antibody
  • NNTM_HUMAN antibody
  • Pyridine nucleotide transhydrogenase antibody
see all

Database links

Anti-NNT antibody [8B4BB10 ] images:

  Flow Cytometry - NNT antibody [8B4BB10 ] (ab110352)

Flow Cytometry - NNT antibody [8B4BB10 ] (ab110352)

HL-60 cells were stained with 1 µg/mL NNT antibody (ab110352) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

  Immunocytochemistry/ Immunofluorescence - Anti-NNT antibody [8B4BB10 ] (ab110352)

Immunocytochemistry/ Immunofluorescence - Anti-NNT antibody [8B4BB10 ] (ab110352)

Mitochondrial localization of NNT in cultured, normal Human skin fibroblasts (strain HDFn) labeled with anti NNT (ab110352) as the primary antibody and an AlexaFluor® 594 goat anti-mouse IgG1 secondary antibody.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NNT antibody [8B4BB10 ] (ab110352)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NNT antibody [8B4BB10 ] (ab110352)

NNT immunohistochemistry in Human cerebellum visualized with ab110352 at 1/100. NNT immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

  Immunoprecipitation - NNT antibody [8B4BB10 ] (ab110352)

Immunoprecipitation - NNT antibody [8B4BB10 ] (ab110352)

Nicotinamide nucleotide transhydrogenase (NNT) immunocaptured from Human liver mitochondria (lane 1), bovine heart mitochondria (lane 2), rat liver mitochondria (lane 3), mouse liver mitochondria (lane 4), and HepG2 cells (lane 5), using ab110352.

  Western blot - NNT antibody [8B4BB10 ] (ab110352)

Western blot - NNT antibody [8B4BB10 ] (ab110352)

Anti-NNT antibody [8B4BB10 ] (ab110352) at 0.5 µg/ml + HepG2 whole cell lysate

Predicted band size : 114 kDa


anti-Complex II 30kDa subunit has also been labeled as a control.

References for Anti-NNT antibody [8B4BB10 ] (ab110352)

ab110352 has not yet been referenced specifically in any publications.

Publishing research using ab110352? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"