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Read our guarantee »Products:Signal Transduction >> Growth Factors/Hormones >> TGF
Anti-NOMO1 antibody
See all NOMO1 products (2) ...
Rabbit polyclonal to NOMO1
WB, ELISAmore details
Reacts with
Human
Synthetic phospho-peptide corresponding to amino acid residues surrounding S1205 of human NOMO1, conjugated to KLH.
293 cell lysate transiently transfected with NOMO1 gene.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS
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Immunogen affinity purified
This antibody is purified through a protein A column, followed by peptide affinity purification.
Polyclonal
IgG
Developmental Biology >> Embryogenesis >> Axis formation and symmetry
Signal Transduction >> Growth Factors/Hormones >> TGF
Western blot - NOMO1 (phospho S1205) antibody (ab75951)
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Our Abpromise guarantee covers the use of ab75951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100.Predicted molecular weight: 134 kDa.
ELISA: 1/1000
May antagonize Nodal signaling.
Expressed in colon tumor tissue and in adjacent normal colonic mucosa.
No difference between normal colonic mucosa and colon tumor tissue in mRNA expression, whereas the protein is expressed 1.5-fold more in normal colonic mucosa that in colon tumor tissue.
Membrane.
Target information above from: UniProt accessionQ15155
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - NOMO1 (phospho S1205) antibody (ab75951)

All lanes : Anti-NOMO1 antibody (ab75951) at 1/50 dilution
Lane 1 : 293 cell lysate, non transfected
Lane 2 : 293 cell lysate transiently transfected with the NOMO1 gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 134 kDa
Observed band size : >130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.
ab75951 has not yet been referenced specifically in any publications.
Publishing research using ab75951? Please let us know so that we can cite the reference in this datasheet
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Concentration not available for this lot.
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All lanes : Anti-NOMO1 antibody (ab75951) at 1/50 dilution
Lane 1 : 293 cell lysate, non transfected
Lane 2 : 293 cell lysate transiently transfected with the NOMO1 gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 134 kDa
Observed band size : >130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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