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Anti-NOMO1 antibody (ab75951)

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Overview

Product name

Anti-NOMO1 antibody
See all NOMO1 products (2) ...

Description

Rabbit polyclonal to NOMO1

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding S1205 of human NOMO1, conjugated to KLH.

Positive control

293 cell lysate transiently transfected with NOMO1 gene.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

This antibody is purified through a protein A column, followed by peptide affinity purification.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - NOMO1 (phospho S1205) antibody (ab75951)Western blot - NOMO1 (phospho S1205) antibody (ab75951) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/50 - 1/100.Predict...Read more →

    WB: 1/50 - 1/100.Predicted molecular weight: 134 kDa.

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

Target

Function

May antagonize Nodal signaling.

Tissue specificity

Expressed in colon tumor tissue and in adjacent normal colonic mucosa.

Developmental stage

No difference between normal colonic mucosa and colon tumor tissue in mRNA expression, whereas the protein is expressed 1.5-fold more in normal colonic mucosa that in colon tumor tissue.

Cellular localization

Membrane.

Target information above from: UniProt accessionQ15155 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Nodal modulator 1 antibody
  • NOMO 1 antibody
  • Nomo antibody
  • NOMO1 antibody
  • NOMO1_HUMAN antibody
  • PM5 antibody
  • pM5 protein antibody
  • pM5 protein telomeric copy antibody
see all

Anti-NOMO1 antibody images:

  Western blot - NOMO1 (phospho S1205) antibody (ab75951)

Western blot - NOMO1 (phospho S1205) antibody (ab75951)

All lanes : Anti-NOMO1 antibody (ab75951) at 1/50 dilution

Lane 1 : 293 cell lysate, non transfected
Lane 2 : 293 cell lysate transiently transfected with the NOMO1 gene

Lysates/proteins at 2 µg per lane.


Predicted band size : 134 kDa
Observed band size : >130 kDa (why is the actual band size different from the predicted?)
Additional bands at : 67 kDa. We are unsure as to the identity of these extra bands.

References for Anti-NOMO1 antibody (ab75951)

ab75951 has not yet been referenced specifically in any publications.

Publishing research using ab75951? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"