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Overview

  • Product nameAnti-nNOS (neuronal) antibodySee all nNOS (neuronal) primary antibodies ...
  • Description
    Rabbit polyclonal to nNOS (neuronal)
  • SpecificityDetects nNOS from bovine, mouse, rabbit and rat tissues. This antibody does not detect endothelial NOS (eNOS) or inducible NOS (iNOS).
  • Tested applicationsICC/IF, IP, IHC-Fr, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide: TKRRAIGFKKLAEAVK-C, corresponding to amino acids 724-739 of Rat nNOS (neuronal).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab3511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use at an assay dependent concentration. PubMed: 22872659
IP IP: Use at an assay dependent concentration.
IHC-Fr IHC-Fr: 1/800.
WB WB: 1/1000.

Target

  • FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • Tissue specificityIsoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • Sequence similaritiesBelongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • DomainThe PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • Post-translational
    modifications    Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • Cellular localizationCell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.

    • Target information above from: UniProt accession P29475 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      2310005C01Rik antibodybNOS antibodyConstitutive NOS antibody
      EC 1.14.13.39 antibodyIHPS 1 antibodyIHPS1 antibodyN NOS antibodyN-NOS antibodyNC NOS antibodyNC-NOS antibodyNCNOS antibodyneuronal Nitric Oxide Synthase antibodyNeuronal NOS antibodyNitric oxide synthase , neuronal, included antibodyNitric oxide synthase 1 (neuronal) antibodyNitric oxide synthase 1 antibodyNitric oxide synthase brain antibodyNitric oxide synthase, brain antibodyNitric oxide synthase, penile neuronal, included antibodynNOS antibodyNO antibodyNOS 1 antibodyNOS antibodyNOS type I antibodyNOS-I antibodyNOS1 antibodyNOS1_HUMAN antibodyPeptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Anti-nNOS (neuronal) antibody images

  • Immunohistochemistry (Frozen sections) - nNOS (neuronal) antibody (ab3511)
    Immunohistochemistry (Frozen sections) - nNOS (neuronal) antibody (ab3511)

    ab3511 at 1/800 dilution staining nNOS (neuronal) in rat brain tissue section by immunohistochemistry (Frozen sections).

References for Anti-nNOS (neuronal) antibody (ab3511)

This product has been referenced in:

See 1 Publication for this product

Publishing research using ab3511 ? Please let us know so that we can cite the reference in this datasheet

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with regard to the antibodies that have been tested and guaranteed (especially for ab72428), what rabbit tissues were used, and what was the localization of the staining? using the same tissue will help to at least confirm the reported reactivity. Aside f...

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Thank you for your reply.

As for ab72428, I will need to check with the lab regarding tested tissue,localization of staining and potential cross-reactivity with eNOS and iNOS. I will letyou know what I find out. Thank you for your patience. Read More

Inquiry: I am interested in purchasing an nNOS antibody for use in immunohistochemistry/immunofluorescence in rabbit paraffin sections. A number of your antibodies in your catalog state they have reactivity with rabbit, and have been documented for use in...

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Thank you for contacting us.

We have 3 antibodies that were tested and are guaranteed (http://www.abcam.com/Abpromise) for IHC-P and rabbit samples: ab1376,ab6175 and ab72428. I would recommend trying one of thosethat were tested as they are co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"