Anti-NOTCH4 antibody - C-terminal (ab199295)
Key features and details
- Rabbit polyclonal to NOTCH4 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-NOTCH4 antibody - C-terminal
See all NOTCH4 primary antibodies -
Description
Rabbit polyclonal to NOTCH4 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NOTCH4 (C terminal). NP_004548
Database link: Q99466 -
Positive control
- Human liver cancer and kidney tissue lysates. Human colon cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 210 kDa.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 210 kDa. |
Target
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Function
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. -
Tissue specificity
Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. -
Sequence similarities
Belongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 28 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats. -
Post-translational
modificationsSynthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
Phosphorylated. -
Cellular localization
Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4855 Human
- Omim: 164951 Human
- SwissProt: Q99466 Human
- Unigene: 436100 Human
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Alternative names
- FLJ16302 antibody
- hNotch 4 antibody
- hNotch4 antibody
see all
Images
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All lanes : Anti-NOTCH4 antibody - C-terminal (ab199295) at 1/500 dilution
Lane 1 : Human liver cancer lysate
Lane 2 : Human kidney lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 210 kDa
Exposure time: 2 minutesGel: 8% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling NOTCH4 using ab199295 at 1/30 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab199295 has been referenced in 3 publications.
- Du Z et al. SOX8 Knockdown Overcomes Enzalutamide Resistance in Castration-Resistant Prostate Cancer by Inhibiting the Notch Signaling Pathway. Biomed Res Int 2022:9235837 (2022). PubMed: 36246971
- Kim DC et al. P2Y2R has a significant correlation with Notch-4 in patients with breast cancer. Oncol Lett 20:647-654 (2020). PubMed: 32565989
- Kikuchi H et al. Numb has distinct function in lung adenocarcinoma and squamous cell carcinoma. Oncotarget 9:29379-29391 (2018). PubMed: 30034624