Anti-NOX1 antibody (ab121009)
Key features and details
- Goat polyclonal to NOX1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NOX1 antibody
See all NOX1 primary antibodies -
Description
Goat polyclonal to NOX1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla -
Immunogen
Synthetic peptide:
DKATDIVTGLKQK
with a Cysteine residue linker, corresponding to internal sequence amino acids 490-502 of Human NOX1 (NP_008983.2) or 441-453 of Human NOX1 (NP_039249.1). -
Positive control
- WB: HeLa, CaCo-2, A549 and U937 cell lysates; IHC-P: Human kidney tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab121009 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. -
Tissue specificity
NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. -
Sequence similarities
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
Cellular localization
Cell projection > invadopodium membrane. - Information by UniProt
-
Database links
- Entrez Gene: 492016 Dog
- Entrez Gene: 27035 Human
- Entrez Gene: 701376 Rhesus monkey
- Omim: 300225 Human
- SwissProt: Q9Y5S8 Human
- Unigene: 592227 Human
-
Alternative names
- GP91 2 antibody
- Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
- Mitogenic oxidase 1 antibody
see all
Images
-
All lanes : Anti-NOX1 antibody (ab121009) at 0.5 µg/ml
Lane 1 : CaCo-2 cell lysate
Lane 2 : CaCo-2 + peptide cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HeLa + peptide cell lysate
Lane 5 : A549 cell lysate
Lane 6 : A549 + peptide cell lysate
Lane 7 : U937 cell lysate
Lane 8 : U937 + peptide cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 65 kDaLysates in RIPA buffer. Detected by chemiluminescence.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling NOX1 with ab121009 at 7.5 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer pH 6. An HRP-conjugated secondary antibody was used. Counterstained with hematoxylin.
Inset: Negative control showing staining of paraffin embedded human kidney, with no primary antibody.
Datasheets and documents
-
Datasheet download
References (8)
ab121009 has been referenced in 8 publications.
- Jing Cao et al. Peroxidasin promotes diabetic vascular endothelial dysfunction induced by advanced glycation end products via NOX2/HOCl/Akt/eNOS pathway. Redox Biol 45:102031 (2021). PubMed: 34116361
- Wang Q et al. A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK-2 cells via regulating NADPH/oxidase-TGF-β/Smad signaling pathway. Phytother Res 35:5227-5240 (2021). PubMed: 34236110
- Zhang W et al. GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis. Aging (Albany NY) 13:22588-22610 (2021). PubMed: 34584017
- Yajun W et al. Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone. Front Pharmacol 12:723988 (2021). PubMed: 34658862
- Jung HY et al. NOX1 Inhibition Attenuates Kidney Ischemia-Reperfusion Injury via Inhibition of ROS-Mediated ERK Signaling. Int J Mol Sci 21:N/A (2020). PubMed: 32967113
- De Bessa TC et al. Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas. Cell Death Dis 10:143 (2019). PubMed: 30760703
- Alborzinia H et al. Golgi stress mediates redox imbalance and ferroptosis in human cells. Commun Biol 1:210 (2018). PubMed: 30511023
- Chocry M et al. Reversion of resistance to oxaliplatin by inhibition of p38 MAPK in colorectal cancer cell lines: involvement of the calpain / Nox1 pathway. Oncotarget 8:103710-103730 (2017). PubMed: 29262595