Recombinant Anti-NPHS2 antibody [EPR13820] (ab181143)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13820] to NPHS2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NPHS2 antibody [EPR13820]
See all NPHS2 primary antibodies -
Description
Rabbit monoclonal [EPR13820] to NPHS2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal kidney lysate; Human kidney tissue, human skeletal muscle tissue. Rat and mouse kidney.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13820 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
For unpurified use at 1/10000 - 1/50000. |
Notes |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). For unpurified use at 1/10000 - 1/50000. |
Target
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Function
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. -
Tissue specificity
Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. -
Involvement in disease
Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2) [MIM:600995]. It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. -
Sequence similarities
Belongs to the band 7/mec-2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7827 Human
- Entrez Gene: 170484 Mouse
- Entrez Gene: 170672 Rat
- Omim: 604766 Human
- SwissProt: Q9NP85 Human
- SwissProt: Q91X05 Mouse
- SwissProt: Q8K4G9 Rat
- Unigene: 412710 Human
see all -
Alternative names
- nephrosis 2 antibody
- Nephrosis 2, idiopathic, steroid resistant (podocin) antibody
- nephrosis 2, idiopathic, steroid resistant antibody
see all
Images
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Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/10000 dilution + Human fetal kidney at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 42 kDa -
Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/50000 dilution (unpurified) + Human fetal kidney tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
All lanes : Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/2000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 42 kDa
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands. -
ab181143 staining NPHS2 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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ab181143 staining NPHS2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NPHS2 with unpurified ab181143 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (15)
ab181143 has been referenced in 15 publications.
- Jiang L et al. METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy. Mol Ther 30:1721-1740 (2022). PubMed: 34995800
- Feng Y et al. Activation of TRPC6 by AngⅡ Induces Podocyte Injury and Participates in Proteinuria of Nephrotic Syndrome. Front Pharmacol 13:915153 (2022). PubMed: 35991898
- Pan X et al. HOXA11-OS participates in lupus nephritis by targeting miR-124-3p mediating Cyr61 to regulate podocyte autophagy. Mol Med 28:138 (2022). PubMed: 36418932
- Dong Z et al. Inhibition of the Wnt/ß-catenin signaling pathway reduces autophagy levels in complement treated podocytes. Exp Ther Med 22:737 (2021). PubMed: 34055054
- Zhu H et al. FOXA1 Suppresses SATB1 Transcription and Inactivates the Wnt/β-Catenin Pathway to Alleviate Diabetic Nephropathy in a Mouse Model. Diabetes Metab Syndr Obes 14:3975-3987 (2021). PubMed: 34531670