If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab28947? Please let us know so that we can cite the reference in this datasheet
ab28947 has been referenced in 18 publications.
Publishing research using ab28947? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Human breast cancer tissue stained with ab28947 NQO1 antibody.
Lane 1 : Marker
Lane 2 : Anti-NQO1 antibody [A180] (ab28947) at 1 µg/ml
Lane 1 : As above
Lane 2 : Kidney (Human) Tissue Lysate (ab7920) at 20 µg
Secondary
Lane 2 : IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size : 30 kDa
Observed band size : 30 kDa
ICC/IF image of ab28947 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab28947, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Sandwich ELISA for the detection of NQO1. ab28947 (1/500) was used as the capture antibody. A rabbit polyclonal raised againts the C-terminal end of NQO1 was used for the detection. Please refer to abreview for further experimental details.
This image is courtesy of an anonymous Abreview
Sandwich ELISA for the detection of NQO1, using ab28947 (1/500) as the capture antibody and ab34173 (1/1000) for the detection.
Overlay histogram showing HeLa cells stained with ab28947 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28947, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
2
Call 01223 696 000 or contact us