Anti-NQO1 antibody [A180] (ab28947)

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - NQO1 antibody [A180] (ab28947)

Human breast cancer tissue stained with ab28947 NQO1 antibody.

  Western blot - NQO1 antibody [A180] (ab28947)

Lane 1 : Marker
Lane 2 : Anti-NQO1 antibody [A180] (ab28947) at 1 µg/ml

Lane 1 : As above
Lane 2 : Kidney (Human) Tissue Lysate (ab7920) at 20 µg

Secondary
Lane 2 : IRDye 680 Conjugated Goat Anti-Mouse IgG (H+L) at 1/10000 dilution


Predicted band size : 30 kDa
Observed band size : 30 kDa

  Immunocytochemistry/ Immunofluorescence - NQO1 antibody [A180] (ab28947)

ICC/IF image of ab28947 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab28947, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  Sandwich ELISA - NQO1 antibody [A180] (ab28947)

Sandwich ELISA for the detection of NQO1. ab28947 (1/500) was used as the capture antibody. A rabbit polyclonal raised againts the C-terminal end of NQO1 was used for the detection. Please refer to abreview for further experimental details.

This image is courtesy of an anonymous Abreview

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  Sandwich ELISA - NQO1 antibody [A180] (ab28947)

Sandwich ELISA for the detection of NQO1, using ab28947 (1/500) as the capture antibody and ab34173 (1/1000) for the detection.