Anti-NQO1 antibody - C-terminal (ab219769)
Key features and details
- Goat polyclonal to NQO1 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-NQO1 antibody - C-terminal
See all NQO1 primary antibodies -
Description
Goat polyclonal to NQO1 - C-terminal -
Host species
Goat -
Specificity
ab219769 is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1). -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Dog, Monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human NQO1 aa 250 to the C-terminus (C terminal) (Cysteine residue). (NP_000894.1).
Database link: P15559 -
Positive control
- Human kidney tissue; human, rat and pig kidney lysates, human lung lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 31 kDa.
|
|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 31 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
Sequence similarities
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 610935 Dog
- Entrez Gene: 101151289 Gorilla
- Entrez Gene: 100067237 Horse
- Entrez Gene: 1728 Human
- Entrez Gene: 18104 Mouse
- Entrez Gene: 100286873 Pig
- Entrez Gene: 100343715 Rabbit
- Entrez Gene: 24314 Rat
see all -
Alternative names
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody - C-terminal (ab219769)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling NQO1 with ab219769 at 5 µg/ml.
-
All lanes : Anti-NQO1 antibody - C-terminal (ab219769) at 1 µg/ml
Lane 1 : Rat kidney lysate (in RIPA buffer)
Lane 2 : Pig kidney lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa -
Anti-NQO1 antibody - C-terminal (ab219769) at 0.03 µg/ml + Human kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 31 kDa -
Anti-NQO1 antibody - C-terminal (ab219769) at 1 µg/ml + Human lung lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 31 kDa
Protocols
Datasheets and documents
-
Datasheet download
References (0)
ab219769 has not yet been referenced specifically in any publications.