NQO1 antibody (ab2346)

Product Name 

NQO1 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to NQO1

Immunogen 

Synthetic peptide: SIPTDNQIKARK, corresponding to C terminal amino acids 267-274 of Human NQO1.
BLAST 'C-SIPTDNQIKARK' with or

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1)

Tested applications 
(see key)

IHC-FoFr, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-FoFr: 1/250 (PMID 19186165).
IHC-Fr: Use at an assay dependent dilution (PMID 17715346).
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Approx 30kDa and 26kDa bands observed in Human Kidney and Human Lung lysates(calculated MW of 30.9kDa, 27.3kDa and 26.4kDa according to NP_000894.1, NP_001020604.1and NP_001020605.1 respecively).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human Kidney and Liver lysate

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Metabolism >> Drug metabolism

Relevance 

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinones thus preventing the formation of reactive oxygen species. Mutations in the gene for NQO1 have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD).
NQO1 levels have been shown to be raised in may tumour types. This has led to a search for anti-tumour agents that can be activated by NQO1 resulting in specific targetting of these compounds to the tumour site. Many cytotoxic drugs contain the quinone moiety, such as the benzoquinone group, which can be activated by NQO1 through the reduction into hydroquinone [which in this case can auto-oxidize and lead to the formation of free radicals and alkylating species]. However, the rat enzyme has been shown to be significantly more effective in activating some of these compounds than the human and mouse enzymes. This indicates that the available cytotoxic drugs are better substrates for the rat enzyme and are not the most ideal prodrugs for activation by NQO1 in human tumors.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: Tris, 0.5% BSA, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Principal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1.
Official Gene Symbol - NQO1.
GenBank Accession Number – NP_000894.
LocusLink ID - 1728.
Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.

See below for NQO1 antibody images, references, products related to ab2346 and other tools.

NQO1 antibody images:

References for NQO1 antibody (ab2346)

  This product has been used in: (two most recent references)

Tan AS & Berridge MV Evidence for NAD(P)H:quinone oxidoreductase 1 (NQO1)-mediated quinone-dependent redox cycling via plasma membrane electron transport: A sensitive cellular assay for NQO1. Free Radic Biol Med 48:421-429 (2010). WB. PubMed: 19932748

Tanaka Y et al. ANIT-induced intrahepatic cholestasis alters hepatobiliary transporter expression via Nrf2-dependent and independent signaling. Toxicol Sci 108:247-57 (2009). PubMed: 19181614

See all 11 publication references for this product.


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