Anti-NQO1 antibody (ab2346)

  Western blot - NQO1 antibody (ab2346)

This image was kindly supplied as part of the review submitted by Lauren Aleksunes. Various mouse liver cytosol samples and human recombinant protein were run on the gel and then incubated with ab2346.

  Western blot - NQO1 antibody (ab2346)

Anti-NQO1 antibody (ab2346) at 0.03 µg/ml + Human Kidney lysate at 35 µg

Observed band size : 30 kDa (why is the actual band size different from the predicted?)


Primary incubation was 1 hour. Detected by chemiluminescence.

  Immunocytochemistry/ Immunofluorescence-NQO1 antibody(ab2346)

ICC/IF image of ab2346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2346, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.