Anti-NQO1 antibody (ab34173)

Western blot - NQO1 antibody (ab34173)

Anti-NQO1 antibody (ab34173) at 1 µg/ml + Human Kidney Whole Tissue Lysate at 20 µg

Secondary
IRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilution

Performed under reducing conditions.

Predicted band size : 31 kDa
Observed band size : 31 kDa

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NQO1 antibody (ab34173)

Image courtesy of Human Protein Atlas . ab34173 staining NQO1 in human breast cancer. Paraffin embedded human breast cancer tissue was incubated with ab34173 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

ab34173 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

Sandwich ELISA - NQO1 antibody (ab34173)

Sandwich ELISA for the detection of NQO1, using ab28947 (1/500) as the cature antibody and ab34173 (1/1000) for the detection. Please refer to abreview for further experimental details.

This image is courtesy of an anonymous Abreview

Western blot

Anti-NQO1 antibody (ab34173) at 1 µg/ml + NQO1 protein (Active) (ab59663) at 0.01 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 30 seconds

Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (ab34173)

ICC/IF image of ab34173 stained HeLa cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34173, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.