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Western blot - NQO1 antibody (ab34173)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NQO1 antibody (ab34173)
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Sandwich ELISA - NQO1 antibody (ab34173)
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Product Name
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NQO1 antibody
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See all NQO1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NQO1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human NQO1.
(Peptide available as ab34172.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat
(Due to sequence homology)
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Tested applications
(see key)
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IHC-P, WB, sELISA
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
sELISA: 1/1000. Can be used as detection antibody in conjunction with ab28947 as capture antibody.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Metabolism >> Drug metabolism
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Relevance
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NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinones thus preventing the formation of reactive oxygen species. Mutations in the gene for NQO1 have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD).
NQO1 levels have been shown to be raised in may tumour types. This has led to a search for anti-tumour agents that can be activated by NQO1 resulting in specific targetting of these compounds to the tumour site. Many cytotoxic drugs contain the quinone moiety, such as the benzoquinone group, which can be activated by NQO1 through the reduction into hydroquinone [which in this case can auto-oxidize and lead to the formation of free radicals and alkylating species]. However, the rat enzyme has been shown to be significantly more effective in activating some of these compounds than the human and mouse enzymes. This indicates that the available cytotoxic drugs are better substrates for the rat enzyme and are not the most ideal prodrugs for activation by NQO1 in human tumors.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NQO1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NQO1 antibody images, references, products related to ab34173 and other tools.
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NQO1 antibody images:
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 Western blot - NQO1 antibody (ab34173)
NQO1 antibody (ab34173) at 1 µg/ml + Human Kidney Whole Tissue Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 31 kDa
Observed band size : 31 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NQO1 antibody (ab34173)
Image courtesy of Human Protein Atlas . ab34173 staining NQO1 in human breast cancer. Paraffin embedded human breast cancer tissue was incubated with ab34173 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab34173 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
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Sandwich ELISA - NQO1 antibody (ab34173)
Sandwich ELISA for the detection of NQO1, using ab28947 (1/500) as the cature antibody and ab34173 (1/1000) for the detection. Please refer to abreview for further experimental details. This image is courtesy of an anonymous Abreview
See Abreview
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References for NQO1 antibody (ab34173)
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This product has been used in: (two most recent references)
Komatsu M et al. The selective autophagy substrate p62 activates the stress responsive transcription factor Nrf2 through inactivation of Keap1. Nat Cell Biol 12:213-23 (2010). WB; Mouse. PubMed: 20173742
Blauwkamp MN et al. Podocyte specific knock out of selenoproteins does not enhance nephropathy in streptozotocin diabetic C57BL/6 mice. BMC Nephrol 9:7 (2008). IHC-P; Mouse. PubMed: 18647412
If you publish research using ab34173 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to NQO1
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NQO1 antibody - more information
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Customer reviews (feedback) regarding NQO1 antibody |
Customer FAQs regarding NQO1 antibody |
Protocols for NQO1 antibody |
Price and availability of products related to NQO1 antibody |
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NQO1 antibody - related products:
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NQO1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NQO1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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