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Products:Neuroscience >> Endocrine system >> Gonadotrophic axis
Overview
Product name
Anti-NR0B1 / Dax1 antibody
See all NR0B1 / Dax1 products (11) ...
Description
Mouse polyclonal to NR0B1 / Dax1
Tested applications
Cross reactivity
Reacts with
Mouse
Immunogen
Fusion protein: SLLTSAQQTHVSREAPEAHRRGEWWQLSYCTQSVGGPEGL QSTQAMAFLYRSYVCGEEQPQQISVASGTPVSADQTPATP QEQPRAPWWDASPGVQRLIT, corresponding to amino acids 150/249 of Mouse NR0B1.
SLLTSAQQTH VSREAPEAHR RGEWWQLSYC TQSVGGPEGL QSTQAMAFLY RSYVCGEEQP QQISVASGTP VSADQTPATP QEQPRAPWWD ASPGVQRLIT
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Orphan Nuclear Receptors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Retinoic & Retinoid
Neuroscience >> Endocrine system >> Hypothalamic pituitary adrenal axis
Neuroscience >> Endocrine system >> Gonadotrophic axis
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Western blot - NR0B1 antibody (ab21884)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 52 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Function
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.
Involvement in disease
Defects in NR0B1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC) [MIM:300200]; also known as X-linked Addison disease (AHX). XL-AHC is a developmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.
Defects in NR0B1 are the cause of 46,XY sex reversal type 2 (SRXY2) [MIM:300018]. It is a condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Note=XY individuals with a duplication of part of the short arm of the X chromosome and an intact SRY gene develop as females. The single X chromosome in these individuals does not undergo X-chromosome inactivation; therefore, these individuals presumably carry 2 active copies of genes, including the NR0B1 gene, in the duplicated region. Individuals with deletion of this region develop as males. Genes within the dosage-sensitive sex reversal region are, therefore, not essential for testis development, but, when present in a double dose, interfere with testis formation.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR0 subfamily.
Domain
Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus.
Target information above from: UniProt accessionP51843
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AHC antibody
- AHCH antibody
- AHX antibody
see all
Database links
- Entrez Gene: 11614 Mouse
- SwissProt: Q61066 Mouse
- Unigene: 5180 Mouse
Anti-NR0B1 / Dax1 antibody images:
Western blot - NR0B1 antibody (ab21884)
All lanes : Anti-NR0B1 / Dax1 antibody (ab21884) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 52 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-NR0B1 / Dax1 antibody (ab21884)
ab21884 has not yet been referenced specifically in any publications.
Publishing research using ab21884? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"