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Western blot - NR0B1 / Dax1 antibody (ab60144)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NR0B1 / Dax1 antibody (ab60144)
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Product Name
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NR0B1 / Dax1 antibody
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See all NR0B1 / Dax1 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NR0B1 / Dax1
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Immunogen
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A region within synthetic peptide: ILYSLLTSSK QTHVAPAAPE ARPGGAWWDR SYFAQRPGGK EALPGGRATA , corresponding to N terminal amino acids 145-194 of Human NR0B1 / Dax1
BLAST 'ILYSLLTSSK QTHVAPAAPE ARPGGAWWDR SYFAQRPGGK EALPGGRATA ' with  or 
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 2.5 µg/ml. Detects bands of approximately 53 and 66 kDa (predicted molecular weight: 53 kDa). We do not know the identity of the 66 kDa band.
ELISA titre using peptide based assay: 1/312500
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate; human intestinal tissue.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Orphan Nuclear Receptors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Retinoic & Retinoid
Neuroscience >> Endocrine system >> hypothalamic pituitary adrenal axis
Neuroscience >> Endocrine system >> Gonadotrophic axis
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Relevance
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NR0B1 is a receptor that may be a component of a cascade required for development of steroidogenic tissues. It acts as a dominant negative regulator of transcription mediated by the retinoic acid receptor.
It interacts with COPS2.
Defects in NR0B1 are the cause of X-linked adrenal hypoplasia congenital (AHC). AHC is a developmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.
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NR0B1 / Dax1 antibody (ab60144) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this NR0B1 / Dax1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for NR0B1 / Dax1 antibody images, references, products related to ab60144 and other tools.
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NR0B1 / Dax1 antibody images:
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 Western blot - NR0B1 / Dax1 antibody (ab60144)
NR0B1 / Dax1 antibody (ab60144) at 2.5 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP-conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 66 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NR0B1 / Dax1 antibody (ab60144)
Immunohistochemical analysis of paraffin-embedded human intestinal tissue using ab60144 at a dilution of 4-8µg/ml, positively labeling epithelial cells of intestinal villus (indicated with arrows). Magnification 400X
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References for NR0B1 / Dax1 antibody (ab60144)
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This product has been used in:
Oda T et al. Tumorigenic role of orphan nuclear receptor NR0B1 in lung adenocarcinoma. Am J Pathol 175:1235-45 (2009). WB, IHC-P; Human, Mouse. PubMed: 19644015
If you publish research using ab60144 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to NR0B1 / Dax1
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NR0B1 / Dax1 antibody - more information
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NR0B1 / Dax1 antibody - related products:
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NR0B1 / Dax1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NR0B1 / Dax1 antibody |
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