NR0B1 / Dax1 antibody (ab60144)

Product Name 

NR0B1 / Dax1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to NR0B1 / Dax1

Immunogen 

A region within synthetic peptide: ILYSLLTSSK QTHVAPAAPE ARPGGAWWDR SYFAQRPGGK EALPGGRATA , corresponding to N terminal amino acids 145-194 of Human NR0B1 / Dax1
BLAST 'ILYSLLTSSK QTHVAPAAPE ARPGGAWWDR SYFAQRPGGK EALPGGRATA ' with or

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 2.5 µg/ml. Detects bands of approximately 53 and 66 kDa (predicted molecular weight: 53 kDa). We do not know the identity of the 66 kDa band.

ELISA titre using peptide based assay: 1/312500

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cell lysate; human intestinal tissue.

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Orphan Nuclear Receptors
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Retinoic & Retinoid
Neuroscience >> Endocrine system >> hypothalamic pituitary adrenal axis
Neuroscience >> Endocrine system >> Gonadotrophic axis

Relevance 

NR0B1 is a receptor that may be a component of a cascade required for development of steroidogenic tissues. It acts as a dominant negative regulator of transcription mediated by the retinoic acid receptor.
It interacts with COPS2.

Defects in NR0B1 are the cause of X-linked adrenal hypoplasia congenital (AHC). AHC is a developmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for NR0B1 / Dax1 antibody images, references, products related to ab60144 and other tools.

NR0B1 / Dax1 antibody images:

References for NR0B1 / Dax1 antibody (ab60144)

  This product has been used in:

Oda T et al. Tumorigenic role of orphan nuclear receptor NR0B1 in lung adenocarcinoma. Am J Pathol 175:1235-45 (2009). WB, IHC-P; Human, Mouse. PubMed: 19644015

If you publish research using ab60144 please let us know so that we can cite the reference on this datasheet.

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