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Products:Neuroscience >> Endocrine system >> Gonadotrophic axis
Overview
Product name
Anti-NR0B1 / Dax1 antibody
See all NR0B1 / Dax1 products (11) ...
Description
Rabbit polyclonal to NR0B1 / Dax1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Chimpanzee
Immunogen
A region within synthetic peptide: ILYSLLTSSK QTHVAPAAPE ARPGGAWWDR SYFAQRPGGK EALPGGRATA , corresponding to N terminal amino acids 145-194 of Human NR0B1 / Dax1
ILYSLLTSSK QTHVAPAAPE ARPGGAWWDR SYFAQRPGGK EALPGGRATA
Positive control
Jurkat cell lysate; human intestinal tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Orphan Nuclear Receptors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Retinoic & Retinoid
Neuroscience >> Endocrine system >> Hypothalamic pituitary adrenal axis
Neuroscience >> Endocrine system >> Gonadotrophic axis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2.5 µg/mlDetects a band of approximately 53, 66 kDa (predicted molecular weight: 53 kDa).Can be blocked with NR0B1 / Dax1 peptide (ab103536). (We do not know the identity of the 66 kDa band.)
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Function
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.
Involvement in disease
Defects in NR0B1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC) [MIM:300200]; also known as X-linked Addison disease (AHX). XL-AHC is a developmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.
Defects in NR0B1 are the cause of 46,XY sex reversal type 2 (SRXY2) [MIM:300018]. It is a condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Note=XY individuals with a duplication of part of the short arm of the X chromosome and an intact SRY gene develop as females. The single X chromosome in these individuals does not undergo X-chromosome inactivation; therefore, these individuals presumably carry 2 active copies of genes, including the NR0B1 gene, in the duplicated region. Individuals with deletion of this region develop as males. Genes within the dosage-sensitive sex reversal region are, therefore, not essential for testis development, but, when present in a double dose, interfere with testis formation.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR0 subfamily.
Domain
Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus.
Target information above from: UniProt accessionP51843
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AHC antibody
- AHCH antibody
- AHX antibody
see all
Database links
- Entrez Gene: 450140 Chimpanzee
- Entrez Gene: 190 Human
- Entrez Gene: 11614 Mouse
- Omim: 300473 Human
- SwissProt: Q9BG97 Chimpanzee
- SwissProt: P51843 Human
- SwissProt: Q61066 Mouse
- Unigene: 268490 Human
see all
Anti-NR0B1 / Dax1 antibody images:
Western blot - NR0B1 / Dax1 antibody (ab60144)
Anti-NR0B1 / Dax1 antibody (ab60144) at 2.5 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP-conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 66 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NR0B1 / Dax1 antibody (ab60144)
Immunohistochemical analysis of paraffin-embedded human intestinal tissue using ab60144 at a dilution of 4-8µg/ml, positively labeling epithelial cells of intestinal villus (indicated with arrows). Magnification 400X
References for Anti-NR0B1 / Dax1 antibody (ab60144)
This product has been referenced in:
- Oda Tet al. Tumorigenic role of orphan nuclear receptor NR0B1 in lung adenocarcinoma. Am J Pathol 175:1235-45 (2009). WB, IHC-P; Human, Mouse.Read more (PubMed: 19644015) »
See 1 publication for this product
Publishing research using ab60144? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"