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Products:Neuroscience >> Endocrine system >> Gonadotrophic axis
Overview
Product name
Anti-NR0B1 / Dax1 antibody
See all NR0B1 / Dax1 products (11) ...
Description
Goat polyclonal to NR0B1 / Dax1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chimpanzee, Rhesus monkey, Marmoset (common), Orangutan
Immunogen
Synthetic peptide: CGEDHPQQGSTLY, corresponding to internal sequence amino acids 202-214 of Human NR0B1 / Dax1 (NP_000466.2)
CGEDHPQQGS TLY
Positive control
Human ovary or testis lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Orphan Nuclear Receptors
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Retinoic & Retinoid
Neuroscience >> Endocrine system >> Hypothalamic pituitary adrenal axis
Neuroscience >> Endocrine system >> Gonadotrophic axis
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Western blot - NR0B1 / Dax1 antibody (ab77260)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab77260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1/32000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.
Involvement in disease
Defects in NR0B1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC) [MIM:300200]; also known as X-linked Addison disease (AHX). XL-AHC is a developmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.
Defects in NR0B1 are the cause of 46,XY sex reversal type 2 (SRXY2) [MIM:300018]. It is a condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Note=XY individuals with a duplication of part of the short arm of the X chromosome and an intact SRY gene develop as females. The single X chromosome in these individuals does not undergo X-chromosome inactivation; therefore, these individuals presumably carry 2 active copies of genes, including the NR0B1 gene, in the duplicated region. Individuals with deletion of this region develop as males. Genes within the dosage-sensitive sex reversal region are, therefore, not essential for testis development, but, when present in a double dose, interfere with testis formation.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR0 subfamily.
Domain
Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus.
Target information above from: UniProt accessionP51843
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AHC antibody
- AHCH antibody
- AHX antibody
see all
Database links
- Entrez Gene: 450140 Chimpanzee
- Entrez Gene: 190 Human
- Entrez Gene: 704030 Rhesus monkey
- Omim: 300473 Human
- SwissProt: Q9BG97 Chimpanzee
- SwissProt: P51843 Human
- SwissProt: Q9BG96 Orangutan
- SwissProt: Q9BG93 Rhesus monkey
see all
Anti-NR0B1 / Dax1 antibody images:
Western blot - NR0B1 / Dax1 antibody (ab77260)
Anti-NR0B1 / Dax1 antibody (ab77260) at 0.5 µg/ml + Human testis lysate in RIPA buffer at 35 µg
Predicted band size : 53 kDa
Observed band size : 53 kDa
References for Anti-NR0B1 / Dax1 antibody (ab77260)
ab77260 has not yet been referenced specifically in any publications.
Publishing research using ab77260? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"