Anti-TR4 antibody (ab137582)
Key features and details
- Rabbit polyclonal to TR4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TR4 antibody
See all TR4 primary antibodies -
Description
Rabbit polyclonal to TR4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human TR4 aa 62-356.
Database link: P49116 -
Positive control
- HeLa, H1299 and Molt-4 cells. NCIN87 Xenograft.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 65 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Developmental stage
Transiently repressed during the meiotic phase of spermatogenesis. -
Post-translational
modificationsPhosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 7182 Human
- Entrez Gene: 22026 Mouse
- Entrez Gene: 50659 Rat
- Omim: 601426 Human
- SwissProt: P49116 Human
- SwissProt: P49117 Mouse
- SwissProt: P55094 Rat
- Unigene: 555973 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- hTAK1 antibody
- Nr2c2 antibody
- NR2C2_HUMAN antibody
see all
Images
-
All lanes : Anti-TR4 antibody (ab137582) at 1/500 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 65 kDa
7.5% SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft labelling TR4 with ab137582 at 1/100 dilution.
-
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labelling TR4 with ab137582 at 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab137582 has been referenced in 2 publications.
- Zhang Y et al. Role and mechanism of miR-4778-3p and its targets NR2C2 and Med19 in cervical cancer radioresistance. Biochem Biophys Res Commun 508:210-216 (2019). PubMed: 30473219
- Zhang L et al. HMGB1 enhances mechanical stress-induced cardiomyocyte hypertrophy in vitro via the RAGE/ERK1/2 signaling pathway. Int J Mol Med 44:885-892 (2019). PubMed: 31524228