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NR2E1 / Tailless protein (Tagged-His Tag) (ab91971)

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Overview

  • Product nameNR2E1 / Tailless protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 49-385 (337 amino acids) of Human NR2E1 / Tailless with an N terminal His tag. Predicted MWt: 38 kDa; UniProt ID = Q9Y466.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 98 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesTYVCKSGNQGGCPVDKTHRNQCRACRLKKCLEVNMNKDAV QHERGPRTSTIRKQVAL YFRGHKEENGAAAHFPSAALP APAFFTAVTQLEPHGLELAAVSTTPERQTLVSLAQP TP KYPHEVNGTPMYLYEVATESVCESAARLLFMSIKWAKSVP AFSTLSLQDQLMLLE DAWRELFVLGIAQWAIPVDANTL LAVSGMNGDNTDSQKLNKIISEIQALQEVVARFR QLRL DATEFACLKCIVTFKAVPTHSGSELRSFRNAAAIAALQDE AQLTLNSYIHTRY PTQPCRFGKLLLLLPALRSISPSTI EEVFFKKTIGNVPITRLLSDMYKSSDI
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91971 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        hTllnr2e1NR2E1_HUMAN
        Nuclear receptor subfamily 2 group E member 1Nuclear receptor TLXOrphan nuclear receptor NR2E1Protein tailless homologTailless homologTllTLXXTLL
      see all
  • FunctionOrphan receptor that binds DNA as a monomer to hormone response elements (HRE) containing an extended core motif half-site sequence 5'-AAGGTCA-3' in which the 5' flanking nucleotides participate in determining receptor specificity. May be required for brain development. May be involved in the regulation of retinal development.
  • Tissue specificityBrain specific. Present in all brain sections tested, highest levels in the caudate nucleus and hippocampus, weakest levels in the thalamus.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q9Y466 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    NR2E1 / Tailless protein (Tagged-His Tag) images

    • SDS-PAGE - NR2E1 / Tailless protein (Tagged-His Tag) (ab91971)
      SDS-PAGE - NR2E1 / Tailless protein (Tagged-His Tag) (ab91971)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for NR2E1 / Tailless protein (Tagged-His Tag) (ab91971)

    ab91971 has not yet been referenced specifically in any publications.

    Publishing research using ab91971 ? Please let us know so that we can cite the reference in this datasheet

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    Hi, I would like to know whether this NR2E1 protein product is in its denatured or native form. Thanks & Regards

    Thank you for your enquiry. The NR2E1 / Tailless protein (Tagged-His Tag) (ab91971) is a recombinant protein so will not be in endogenous native form. However, it will still have secondary and tertiary structure, and possible post translational modifi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"