Anti-NRAS antibody (ab77392)
Key features and details
- Goat polyclonal to NRAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-NRAS antibody
See all NRAS primary antibodies -
Description
Goat polyclonal to NRAS -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human NRAS aa 150 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Accession Number: NP_002515.1
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Positive control
- WB: MCF7 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab77392 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (6) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
1 hour primary incubation at room temperature is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). 1 hour primary incubation at room temperature is recommended for this product. |
Target
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Function
Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. -
Involvement in disease
Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits. -
Sequence similarities
Belongs to the small GTPase superfamily. Ras family. -
Post-translational
modificationsPalmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 4893 Human
- Entrez Gene: 18176 Mouse
- Entrez Gene: 24605 Rat
- Omim: 164790 Human
- SwissProt: P01111 Human
- SwissProt: P08556 Mouse
- SwissProt: Q04970 Rat
- Unigene: 486502 Human
see all -
Alternative names
- ALPS4 antibody
- AV095280 antibody
- GTPase NRas antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab77392 has been referenced in 12 publications.
- Yan Y et al. NRAS expression is associated with prognosis and tumor immune microenvironment in lung adenocarcinoma. J Cancer Res Clin Oncol 148:565-575 (2022). PubMed: 34746975
- Ma Y et al. Neuronal miR-29a protects from obesity in adult mice. Mol Metab 61:101507 (2022). PubMed: 35490865
- Nam HJ et al. GPR110 promotes progression and metastasis of triple-negative breast cancer. Cell Death Discov 8:271 (2022). PubMed: 35614051
- Bery N et al. A potent KRAS macromolecule degrader specifically targeting tumours with mutant KRAS. Nat Commun 11:3233 (2020). PubMed: 32591521
- Dey S et al. miR-29a Is Repressed by MYC in Pancreatic Cancer and Its Restoration Drives Tumor-Suppressive Effects via Downregulation of LOXL2. Mol Cancer Res 18:311-323 (2020). PubMed: 31662451