Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-NRG1 type III antibody
Description
Rabbit polyclonal to NRG1 type III
Specificity
ab23248 detects ~50-55kDa band in rat sciatic nerve, rat and mouse brain lysate. IHC detection with ab23248 in mouse DRG shows a primarily nuclear localisation of the protein rather than the expected cytoplasmic staining (See Bao and Wang references).
Tested applications
IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Chicken, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human NRG1 type III.
(Peptide available as ab233 78.)
Positive control
mouse L4/5 dorsal root ganglion (DRG) tissue. Expected positive: mouse/rat sciatic nerve; neural crest derived neuroblasts
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr
- 1 Image
WB
Application notes
IHC-Fo: Use at a concentration of 0.34 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately ~52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
NRG1 type III is a sensory and motor neuron-derived factor isoform of Neuregulin 1, specifically expressed in the nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. NRG1 type III is the predominant isoform expressed in sensory and motor neurons. The Nrg1 gene encodes more than 15 transmembrane and secreted protein isoforms, generated by alternative promoter usage and mRNA splicing. Nrg1 subtypes I through III share the epidermal growth factor-like signaling domain and are defined by different amino-termini. The N-terminal cysteine-rich domain (CRD), as found in the sensory and motor neuronderived factor (SMDF), defines Nrg1 type III, which in embryonic development is responsible for survival of Schwann cell precursors.
Cellular localization
Cell Membrane and Secreted
Alternative names
- GGF antibody
- Heregulin antibody
- HGL antibody
see all
Database links
- Entrez Gene: 3084 Human
- Entrez Gene: 211323 Mouse
- Entrez Gene: 112400 Rat
- SwissProt: Q02297 Human
- SwissProt: Q15491 Human
- SwissProt: P43322 Rat
Anti-NRG1 type III antibody images:
Immunohistochemistry (PFA fixed) - NRG1 type III antibody (ab23248)
ab23248 detecting NRG1 Type III in mouse L4/5 dorsal root ganglion (DRG) tissue (perfused with 4% paraformaldehyde and cryoprotected overnight, cut on a cryostat at 15µm). Tissue was incubated in blocking serum (normal goat serum) for 1hr, washed 3XPBS before primary antibody incubation in PBS with triton (0.1%) overnight (ab23248 was used at 1/800 = 0.34µg/ml). Secondary antibody used was goat anti-rabbit Alexa 546 (1/1000). The image shown is taken with a X20 objective and shows mostly nuclear NRG1 Type III staining with some cytomplasmic staining (nuclear counterstain with DAPI confirmed this - not shown).
David Bennett, Kings College London, United Kingdom
Western blot - NRG1 type III antibody (ab23248)
All lanes : Anti-NRG1 type III antibody (ab23248) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Lysate
Lane 2 : Mouse Brain
Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 27,52,74,75 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
ab23248 picks up an ~50kDa band in WB when tested on rat sciatic nerve, rat brain or mouse brain. This band size concurrs with an entry for NRG1 in both Expasy and NCBI databases (Q3URY7). Carroll et al., (J Neurosci. 1997 Mar 1;17(5):1642-59) also detect a 50kDa band in dorsal root ganglion and spinal cord. However, it is surprising that we do not detect more bands; it is likely that ab23248 does not pick up all NRG1 type III isoforms or that there is very restricted expression in the tissues tested here.
References for Anti-NRG1 type III antibody (ab23248)
ab23248 has not yet been referenced specifically in any publications.
Publishing research using ab23248? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"