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Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472)

Abreviews (1)Q&A (2)

Overview

  • Product nameAnti-NSE antibody [1C1] (HRP) - Neuronal MarkerSee all NSE primary antibodies ...
  • Description
    Mouse monoclonal [1C1] to NSE - Neuronal Marker (HRP)
  • ConjugationHRP
  • SpecificityReacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
  • Tested applicationsELISA, Sandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Neuron Specific Enolase from human brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab24472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution.
Sandwich ELISA sELISA: Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5A4] to NSE - Neuronal Marker (ab10169) and Mouse monoclonal [5E2] to NSE - Neuronal Marker (ab10171) and Mouse monoclonal [5G10] to NSE - Neuronal Marker (ab8324). Can be used as detection antibody in conjunction with recommended pair(s).
WB WB: 1/1000.

Target

  • FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similaritiesBelongs to the enolase family.
  • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

    • Target information above from: UniProt accession P09104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        2 phospho D glycerate hydrolyase antibody2-phospho-D-glycerate hydro-lyase antibodyEno 2 antibody
        Eno2 antibodyENOG antibodyENOG_HUMAN antibodyEnolase 2 antibodyEnolase 2 gamma neuronal antibodyEnolase2 antibodyGamma enolase antibodyGamma-enolase antibodyNeural enolase antibodyNeuron specific enolase antibodyNeuron specific gamma enolase antibodyNeuron-specific enolase antibodyNeurone specific enolase antibodyNSE antibody
      see all

    Anti-NSE antibody [1C1] (HRP) - Neuronal Marker images

    • Western blot - Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472)
      Western blot - Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472)
      All lanes : Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472) at 1/1000 dilution

      Lane 2 : NSE protein (Human) (ab78797) at 5 µg
      Lane 3 : NSE protein (Human) (ab78797) at 0.005 µg
      Lane 4 : Whole tissue lysate prepared from human brain at 15 µg

      developed using the ECL technique

      Observed band size : 45 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Image courtesy of Dr Rachel Barker by Abreview.

      See Abreview

    References for Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472)

    ab24472 has not yet been referenced specifically in any publications.

    Publishing research using ab24472 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab24472 Submit a Question for ab24472

    Displaying 1 - 3 of 3 results for Abreviews and Q&A

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    I read that sodium azide, which is used in the storage buffer for the antibodies ab24472, could inhibit the horseradish peroxidase.

    Do you have any information if in this case there is no inhibition?

    Best regards,

    Thank you for your enquiry.

    I have investigated our records for ab24472 with my colleagues and the originator. I can confirm that regrettably there is an error on the datasheet, and this antibody does not contain sodium azide.

    The da...

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    In december I ordered the antibody ab24472 to use it in an ELISA. The problem is that this antibody does not recognize my standard very well. I tried to coat the microtiter plate with the native gamma enolase (isolated from human brain) and then added dir...

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    Thank you for contacting us. I am sorry to hear that you are having trouble using this antibody.

    I have read the details provided and suspect that the standard may not be pure enough or may be high conc. of . standard is needed. I have further q...

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    Western blot abreview for Anti-NSE antibody [1C1] (HRP) - Neuronal Marker

     Excellent
    Abreviews
    Application Western blot
    Sample Human Tissue lysate - whole (brain)
    Loading amount 15 µg
    Specification brain
    Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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    Verified customer

    Submitted Mar 21 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"