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- Primary antibodies
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Product name
Anti-NSE antibody [5G10] - Neuronal Marker
See all NSE products (29) ...
Description
Mouse monoclonal [5G10] to NSE - Neuronal Marker
Specificity
Reacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
Tested applications
Sandwich ELISA, ICC/IF, ELISA, IHC-FoFr, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Purified Neuron Specific Enolase from human brain
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
5G10
Myeloma
Sp2/0
Isotype
IgG2b
Light chain type
unknown
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Developmental Biology >> Lineage specification >> Ectoderm
Cancer >> Tumor biomarkers >> Enzymes >> Neuron Specific Enolase
Stem Cells >> Lineage Markers >> Ectoderm
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [1C1] to NSE - Neuronal Marker (ab8328). For sandwich ELISA, we recommend using this antibody for capture with ab24472 as detection. Can be used as capture antibody in conjunction with ab8328 and ab24472.
ICC/IF: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [1C1] to NSE - Neuronal Marker (ab8328).
IHC-FoFr: 1/100 - 1/300.
IHC-P: Use at an assay dependent dilution. Recommended dilution for immunohistochemistry on formalin-fixed and paraffin-embedded sections is 1:100 - 1:300. Optimal dilution must be determined by experimentation.
Target
Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Tissue specificity
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Sequence similarities
Belongs to the enolase family.
Developmental stage
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Cellular localization
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Target information above from: UniProt accessionP09104
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
Database links
- Entrez Gene: 2026 Human
- Omim: 131360 Human
- SwissProt: P09104 Human
- Unigene: 511915 Human
References for Anti-NSE antibody [5G10] - Neuronal Marker (ab8324)
This product has been referenced in:
- Dale RC et al. Encephalitis lethargica syndrome: 20 new cases and evidence of basal ganglia autoimmunity. Brain 127:21-33 (2004).Read more (PubMed: 14570817) »
See 1 publication for this product
Publishing research using ab8324? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"