Anti-NSE antibody (ab64721)

Overview

• Product nameAnti-NSE antibodySee all NSE primary antibodies ...
• Description
  Rabbit polyclonal to NSE
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Recombinant full length human NSE purified from E. coli.

• Positive controlRat spinal cord or human embryonic kidney cells line 293.
• General notesThe total protein content in serum: about 60mg/ml.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 10mM Sodium Azide
  Constituents: Whole serum
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Carbohydrate metabolism

  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Cancer
  • Tumor biomarkers
  • Enzymes
  • Neuron Specific Enolase

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of carbohydrates

  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Soma marker

Applications

Target

• FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
• Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
• PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
• Sequence similaritiesBelongs to the enolase family.
• Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
• Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

Target information above from: UniProt accession P09104 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2026 Human
  • Entrez Gene: 24334 Rat
  • Omim: 131360 Human
  • SwissProt: P09104 Human
  • SwissProt: P07323 Rat
  • Unigene: 511915 Human
  • Unigene: 10828 Rat

• Alternative names
  2 phospho D glycerate hydrolyase antibody2-phospho-D-glycerate hydro-lyase antibodyEno 2 antibody

  Eno2 antibodyENOG antibodyENOG_HUMAN antibodyEnolase 2 antibodyEnolase 2 gamma neuronal antibodyEnolase2 antibodyGamma enolase antibodyGamma-enolase antibodyNeural enolase antibodyNeuron specific enolase antibodyNeuron specific gamma enolase antibodyNeuron-specific enolase antibodyNeurone specific enolase antibodyNSE antibody

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Anti-NSE antibody images

• 

  Western blot - NSE antibody (ab64721)
  Anti-NSE antibody (ab64721) at 1/2000 dilution + Rat spinal cord
  
  Predicted band size : 47 kDa
  Observed band size : 47 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - NSE antibody (ab64721)
  Immunofluorescent staining of human embryonic kidney cell line 293 by ab64721 (1:500). Hek293 expresses many neuronal proteins.
  
  The red channel shows staining with ab64721, which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker with a monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1), ab64722. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.