Overview
- Product nameAnti-NSE antibodySee all NSE primary antibodies ...
- DescriptionRabbit polyclonal to NSE
- Tested applicationsWB, ICC/IF more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Non Human Primates, Zebrafish - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human NSE.
(Peptide available as ab99878.)
- Positive controlThis antibody gave a positive signal in the following lysates: HeLa Whole Cell; HepG2 Whole Cell; Hek293 Whole Cell; Jurkat Whole Cell; u87MG Whole Cell; SHSY-5Y Whole Cell; Human Liver Tissue Lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab90473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).Can be blocked with NSE peptide (ab99878). |
| ICC/IF | ICC/IF: Use a concentration of 10 µg/ml. |
Target
- FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
- Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
- PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
- Sequence similaritiesBelongs to the enolase family.
- Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
- Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
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Database links
- Entrez Gene: 395689 Chicken
- Entrez Gene: 526006 Cow
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Entrez Gene: 24334 Rat
- Entrez Gene: 402874 Zebrafish
- Omim: 131360 Human
- SwissProt: O57391 Chicken
- SwissProt: P09104 Human
- SwissProt: P17183 Mouse
- SwissProt: P07323 Rat
- Unigene: 511915 Human
- Unigene: 3913 Mouse
- Unigene: 10828 Rat
see all
Target information above from: UniProt accession
P09104
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 2 phospho D glycerate hydrolyase antibody2-phospho-D-glycerate hydro-lyase antibodyEno 2 antibody
- Eno2 antibodyENOG antibodyENOG_HUMAN antibodyEnolase 2 antibodyEnolase 2 gamma neuronal antibodyEnolase2 antibodyGamma enolase antibodyGamma-enolase antibodyNeural enolase antibodyNeuron specific enolase antibodyNeuron specific gamma enolase antibodyNeuron-specific enolase antibodyNeurone specific enolase antibodyNSE antibody
see all
Anti-NSE antibody images
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Western blot - NSE antibody (ab90473)All lanes : Anti-NSE antibody (ab90473) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 7 :Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes -
Immunocytochemistry/ Immunofluorescence - NSE antibody (ab90473)ICC/IF image of ab90473 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90473 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Formaldehyde fixed (4%, 10min) HeLa, Hek293, and MCF-7 cells
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Western blotAnti-NSE antibody (ab90473) at 1 µg/ml +NSE protein (Human) (ab78797) at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
References for Anti-NSE antibody (ab90473)
ab90473 has not yet been referenced specifically in any publications.
