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Overview

  • Product nameNSE protein (Human)See all NSE proteins and peptides ...
  • Protein descriptionRecombinant full length human NSE; 434 amino acids, 47kDa. Swiss ID = P09104.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurity is > 98% by SDS PAGE. ab78797 is purified using conventional chromatography techniques.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.1M Potassium chloride, 20mM Tris, 5mM Magnesium sulphate, pH 7.5
  • Concentration information loading...
  • Sequence notesMSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab78797 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab78797 can be used as a WB positive control in conjunction with ab90473.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab24709,ab24711,ab90473 and ab166817.

    • Protein info

    • Alternative names
        2 phospho D glycerate hydrolyase2-phospho-D-glycerate hydro-lyaseEno 2
        Eno2ENOGENOG_HUMANEnolase 2Enolase 2 gamma neuronalEnolase2Gamma enolaseGamma-enolaseNeural enolaseNeuron specific enolaseNeuron specific gamma enolaseNeuron-specific enolaseNeurone specific enolaseNSE
      see all
  • FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similaritiesBelongs to the enolase family.
  • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

    • Target information above from: UniProt accession P09104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    NSE protein (Human) images

    • Western blot
      Western blot
      Anti-NSE antibody (ab90473) at 1 µg/ml + NSE protein (Human) (ab78797) at 0.1 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 4 minutes
    • SDS-PAGE - NSE protein (Human) (ab78797)
      SDS-PAGE - NSE protein (Human) (ab78797)
      15% SDS-PAGE showing ab78797 at approximately 47kDa (3µg).

    References for NSE protein (Human) (ab78797)

    ab78797 has not yet been referenced specifically in any publications.

    Publishing research using ab78797 ? Please let us know so that we can cite the reference in this datasheet

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    Recommended conditions for storage of proteins.

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    We recommend storing the protein in 10ul aliquots at -20C for longer term.

    Although we haven't used these proteins in functional assays however you can try using these proteins either by diluting in cultur...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"