Overview

  • Product nameAnti-NSE2 antibody [4A2]See all NSE2 primary antibodies ...
  • Description
    Mouse monoclonal [4A2] to NSE2
  • Tested applicationsWB, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein of Human NSE2 produced in E.coli (NP_775956).

  • Positive control
    • HEK293T cells transfected with pCMV6-ENTRY control and pCMV6-ENTRY NSE2, COS7 cells transiently transfected by pCMV6-ENTRY NSE2, HEK293T cells transfected with overexpress NSE2 plasmid
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab119074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500. Predicted molecular weight: 27 kDa.
Flow Cyt 1/100.
ICC/IF 1/100.

Target

  • FunctionE3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
  • PathwayProtein modification; protein sumoylation.
  • Sequence similaritiesBelongs to the NSE2 family.
    Contains 1 SP-RING-type zinc finger.
  • Post-translational
    modifications
    Sumoylated, possibly via autosumoylation.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession Q96MF7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • C8orf36 antibody
    • E3 SUMO-protein ligase NSE2 antibody
    • hMMS21 antibody
    • hNSE2 antibody
    • methyl methanesulfonate sensitivity gene 21 antibody
    • MMS21 antibody
    • MMS21 homolog antibody
    • non-SMC element 2 antibody
    • Non-SMC element 2 homolog antibody
    • Non-structural maintenance of chromosomes element 2 homolog antibody
    • NSE2_HUMAN antibody
    • Nsmce2 antibody
    see all

Anti-NSE2 antibody [4A2] images

  • All lanes : Anti-NSE2 antibody [4A2] (ab119074) at 1/500 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY NSE2

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 27 kDa
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NSE2 using ab119074 at a dilution of 1/100.
  • HEK293T cells transfected with either NSE2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119074 at a dilution of 1/100, and then analyzed by flow cytometry.

References for Anti-NSE2 antibody [4A2] (ab119074)

ab119074 has not yet been referenced specifically in any publications.

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