Anti-NSE2 antibody [4A2] (ab119074)

Overview

• Product nameAnti-NSE2 antibody [4A2]See all NSE2 primary antibodies ...
• Description
  Mouse monoclonal [4A2] to NSE2
• Tested applicationsWB, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein of Human NSE2 produced in E.coli (NP_775956).

• Positive controlHEK293T cells transfected with pCMV6-ENTRY control and pCMV6-ENTRY NSE2, COS7 cells transiently transfected by pCMV6-ENTRY NSE2, HEK293T cells transfected with overexpress NSE2 plasmid
• General notesDilute in PBS (pH7.3) before use.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 1% BSA, 50% Glycerol
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number4A2
• IsotypeIgG1
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Homologous Recomb.

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Sumo

Applications

Target

• FunctionE3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
• PathwayProtein modification; protein sumoylation.
• Sequence similaritiesBelongs to the NSE2 family.
  Contains 1 SP-RING-type zinc finger.
• Post-translational
  modificationsSumoylated, possibly via autosumoylation.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q96MF7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 286053 Human
  • SwissProt: Q96MF7 Human
  • Unigene: 388297 Human

• Alternative names
  C8orf36 antibodyE3 SUMO-protein ligase NSE2 antibodyhMMS21 antibody

  hNSE2 antibodymethyl methanesulfonate sensitivity gene 21 antibodyMMS21 antibodyMMS21 homolog antibodynon-SMC element 2 antibodyNon-SMC element 2 homolog antibodyNon-structural maintenance of chromosomes element 2 homolog antibodyNSE2_HUMAN antibodyNsmce2 antibody

  see all

Anti-NSE2 antibody [4A2] images

• 

  Western blot - Anti-NSE2 antibody [4A2] (ab119074)
  All lanes : Anti-NSE2 antibody [4A2] (ab119074) at 1/500 dilution
  
  Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
  Lane 2 : HEK293T cells transfected with pCMV6-ENTRY NSE2
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 27 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-NSE2 antibody [4A2] (ab119074)
  Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NSE2 using ab119074 at a dilution of 1/100.
• 

  Flow Cytometry - Anti-NSE2 antibody [4A2] (ab119074)
  HEK293T cells transfected with either NSE2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab119074 at a dilution of 1/100, and then analyzed by flow cytometry.