Overview
- Product nameAnti-NSL1 antibodySee all NSL1 primary antibodies ...
- DescriptionRabbit polyclonal to NSL1
- Tested applicationsIP more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide, corresponding to a region between amino acids 150-200 of Human NSL1 (NP_056286.2)
- Positive controlWhole cell lysate from HeLa cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab70835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IP | IP: Use at 2-5 µg/mg of lysate. |
Target
- FunctionPart of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
- Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. - Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
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Database links
- Entrez Gene: 25936 Human
- Entrez Gene: 25936 Human
- Entrez Gene: 25936 Human
- SwissProt: Q96IY1 Human
- SwissProt: Q96IY1 Human
- SwissProt: Q96IY1 Human
- Unigene: 497692 Human
Target information above from: UniProt accession
Q96IY1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- 4833432M17Rik antibodyAI451597 antibodyC1orf48 antibody
- DC31 antibodyDC8 antibodyDKFZp566O1646 antibodyGm105 antibodyGm984 antibodyKinetochore associated protein NSL1 homolog antibodyKinetochore-associated protein NSL1 homolog antibodyMIS14 antibodyNSL1 antibodyNSL1, MIND kinetochore complex component, homolog (S. cerevisiae) antibodyNSL1_HUMAN antibody
see all
Anti-NSL1 antibody images
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Detection of Human NSL1 by Western Blot of Immunoprecipitates in Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.
Lane 1: ab70835 used for IP at 3 µg/mg lysate.
Lane 2: immunoprecipitation with an other antibody
Lane 3: ab70836 used for IP at 3 µg/mg lysate
Lane 4: Control IgG IP
Detection: Chemiluminescence with an exposure time of 3 minutes. For blotting ab70836 was used at 1 µg/ml.
References for Anti-NSL1 antibody (ab70835)
ab70835 has not yet been referenced specifically in any publications.
