Anti-NSP A + NSP B antibody [RNL-4] (ab8961)

Overview

• Product nameAnti-NSP A + NSP B antibody [RNL-4]
• Description
  Mouse monoclonal [RNL-4] to NSP A + NSP B
• SpecificityThis antibody reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative.
• Tested applicationsFlow Cyt, ICC, IHC-Fr, WB more details
• Species reactivity
  Reacts with: Rat, Human, Pig
  
• Immunogen

  Synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.

• EpitopeThis antibody recognizes an epitope located within the first 20 amino acids of Reticulon-1C (NSP-C).
• General notesReticulon-1 has been found to indicate neuronal differentiation and to be downregulated in neurological pathologies.

  
  

  Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• Primary antibody notes Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
• Clonality Monoclonal
• Clone numberRNL-4
• MyelomaSp2/0-Ag14
• IsotypeIgG1
• Research Areas
  • Neuroscience
  • Neurology process
  • Neuroendocrinology
  • General neuroendocrinology

Applications

Target

• RelevanceReticulon 1 (NSP) may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom.
• Cellular localizationEndoplasmic reticulum membrane
• Database links
  • Entrez Gene: 6252 Human
  • Entrez Gene: 116644 Rat
  • Omim: 600865 Human
  • SwissProt: Q16799 Human
  • SwissProt: Q64548 Rat

• Alternative names
  Neuroendocrine specific protein antibodyNSP antibodyNSPA antibody

  NSPB antibodyReticulon A antibodyReticulon B antibodyReticulon1 antibodyRTN1 antibodyRTN1A antibodyRTN1B antibody

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Anti-NSP A + NSP B antibody [RNL-4] images

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  Schematic representation of of the deduced domain structure of the three NSP-gene encoded proteins, NSP-A, NSP-B and NSP-C and mapping of the epitopes recognized by the different NSP monoclonal antibodies.